桃YABBY转录因子家族的生物信息学分析

Bioinformatic Analyses of YABBY Transcription Factors in Prunus persica

为了解桃中YABBY家族的成员和特征,本研究基于桃(Prunus persica)最新基因组组装注释数据,首次通过生物信息学手段系统鉴定并分析了YABBY基因家族特征,包括染色体定位、蛋白特征、基因结构、进化关系、保守结构域、启动子区域顺式元件。结果显示:桃中含有6个YABBY基因,分布在5条染色体上,其推导蛋白序列较短,分子量较小。家族成员含有4~6个内含子,结构较为复杂。桃中YABBY基因含有植物YABBY家族所有5个亚类,即CRC、INO、FIL/YAB3、YAB2和YAB5,且均含有保守的锌指结构和YABBY结构域。除了核心启动元件,桃YABBY基因还含有大量光应答、激素响应等顺式作用元件,表明该家族基因的表达可能会受到环境、激素等的影响。本研究初步研究了桃基因组中YABBY基因家族的特征,为后期该基因家族在桃中的功能验证提供了理论支撑。

To understand the members and characteristics of YABBY family in peach,based on the latest genome assembly annotation data of Prunus persica,we systematically identified and analyzed YABBY gene family characteristics,including chromosomal localization,protein characteristics,gene structure,evolutionary relationships,conserved domains,promoter region cis-elements by bioinformatics means for the first time.The results show that P.persica contains 6 YABBYs,distributed on 5 chromosomes,its derived protein sequence is short,and molecular weight is small.The YABBYs gene family members contain 4~6 introns and the structure is relatively complex.The YABBYs in peach contain all five subclasses of plant YABBY family,namely,CRC、INO、FIL/YAB3、YAB2 and YAB5,and all contain conserved zinc finger structure and YABBY domain.In addition to the core initiation elements,peach YABBYs also contains a large number of light response,hormone response and other cis-acting elements,indicating that the expression of the family gene may be affected by the environment,hormones,etc.A preliminary study on the characteristics of YABBY gene family in peach genome provides theoretical support for the functional verification of the gene family in P.persica in the later stage.