摘要
将PEDVFJLY1703株ORF3基因进行RT-PCR扩增并进行遗传进化和序列分析,结果表明,FJLY1703株ORF3基因核苷酸序列与2010年以后中国变异株的一致性为97.8%~99.6%,与疫苗株CV777的同源性为95.6%。FJLY1703株与疫苗株CV777亲缘性较远,并存在与疫苗株CV777不同的抗原表位。重组分析表明FJLY1703株ORF3基因没有发生重组事件,且易出现变异。
The ORF3 gene of the PEDVFJLY1703 strain was amplified by RT-PCR,and genetic evolution and sequence analysis were performed.The results showed that the nucleotide sequence of the ORF3 gene of the FJLY1703 strain was 97.8%to 99.6%identical to the Chinese variant strain after 2010,which was comparable to the vaccine strain.The homology of CV777 is 95.6%.The FJLY1703 strain is far from the vaccine strain CV777 and has a different epitope from the vaccine strain CV777.Recombination analysis showed that the ORF3 gene of FJLY1703 strain did not undergo recombination events and was prone to mutation.
作者
董波
戴爱玲
李晓华
杨小燕
DONG Bo;DAI Ai-ling;LI Xiao-hua;YANG Xiao-yan(College of Life Science,Longyan University,Longyan 364012,China;Fujian Engineering Research Center for Swine Disease Control and Prevention,Longyan University,Longyan 364012,China)
出处
《唐山师范学院学报》
2020年第6期59-62,86,共5页
Journal of Tangshan Normal University
关键词
猪流行性腹泻病毒
ORF3
进化特征
Porcine Epidemic Diarrhea Virus
ORF3
evolutionary characteristics