线粒体呼吸链酶复合体活性变化与溃疡性结肠炎的关系

Relationship between the activity of mitochondrial respiratory chain complex and ulcerative colitis

目的观察溃疡性结肠炎(ulcerative colitis,UC)患者肠黏膜线粒体呼吸链酶复合体活性变化,探讨其与UC的关系。方法UC患者180例,在直肠(距肛缘10~15 cm)取病变黏膜组织4块为UC组,在升结肠(距回盲部≤5 cm)取正常黏膜组织4块为UC对照组;结直肠癌筛查中肠镜检查正常者180例,其直肠正常黏膜组织4块为正常对照组。采用ELISA法检测3组黏膜组织三磷酸腺苷(adenosine triphosphate,ATP)含量,采用紫外分光光度法检测3组黏膜组织呼吸链酶复合体Ⅰ~Ⅴ活性。结果UC组肠黏膜组织ATP含量[(1.22±0.37)μmol/g]和呼吸链酶复合体Ⅰ活性[(0.57±0.21)μmol/(min·mg)]明显低于UC对照组[(1.75±0.41)μmol/g、(1.37±0.18)μmol/(min·mg)]和正常对照组[(1.95±0.44)μmol/g、(1.31±0.24)μmol/(min·mg)](P<0.05),UC对照组与正常对照组比较差异无统计学意义(P>0.05);3组肠黏膜组织呼吸链酶复合体Ⅱ、Ⅲ、Ⅳ、Ⅴ活性比较差异均无统计学意义(P>0.05)。轻、中、重度UC患者肠黏膜组织ATP含量[(1.23±0.35)、(1.28±0.23)、(1.17±0.38)μmol/g]和复合体Ⅰ活性[(0.52±0.31)、(0.58±0.16)、(0.49±0.35)μmol/(min·mg)]比较差异均无统计学意义(P>0.05)。UC组肠黏膜组织ATP含量与呼吸链酶复合体Ⅰ活性呈正相关(r=0.890,P=0.017)。结论UC患者病变肠黏膜组织ATP含量和线粒体呼吸链酶复合体Ⅰ活性均明显下降,线粒体呼吸链酶复合体活性下降导致线粒体能量代谢障碍可能是UC的重要发病机制。

Objective To observe the changes of mitochondrial respiratory chain complex activity in patients with ulcerative colitis(UC),and to analyze its relationship with UC.Methods Four specimens from UC mucosa(UC group)were taken from the rectum(10-15 cm away from the anal margin)and four specimens from the normal mucosa(UC control group)were taken from the ascending colon(less than 5 cm from ileocecal region)in 180 UC patients,and another four specimens from normal mucosa(normal control group)were taken from the rectum in 180 subjects with normal colonscopic result in colorectal cancer screeening.The content of adenosine triphosphate(ATP)was detected by ELISA,and the activities of respiratory chain complex Ⅰ to Ⅴ were detected by ultraviolet spectrophotometry in three groups.Results The content of ATP and the activity of respiratory chain complex Ⅰ were lower in UC group((1.22±0.37)μmol/g,(0.57±0.21)μmol/(min·mg))than those in UC control group((1.75±0.41)μmol/g,(1.37±0.18)μmol/(min·mg))and normal control group((1.95±0.44)μmol/g,(1.31±0.24)μmol/(min·mg))(P<0.05),and showed no significant difference between UC control group and normal control group(P>0.05).There were no significant differences in the activities of respiratory chain complex Ⅱ,Ⅲ,Ⅳ and Ⅴ among three groups(P>0.05).The ATP contents((1.23±0.35),(1.28±0.23),(1.17±0.38)μmol/g)and respiratory chain complex Ⅰ activities((0.52±0.31),(0.58±0.16),(0.49±0.35)μmol/(min·mg))showed no significant differences in patients with mild,moderate and severe UC(P>0.05).The ATP content was positively correlated with the activity of respiratory chain complex Ⅰ in UC group(r=0.890,P=0.017).Conclusion The ATP content and mitochondrial respiratory chain complex Ⅰ activity in UC mucosa decrease significantly in UC patients.The decrease of mithochondrial respiratory chain complex activity would lead to mitochondrial energy metabolism disorder,which might be an important pathogenesis of UC.