GPC-1、miRNA-9及Caspase-3在肺腺癌中的表达及与病情和预后生存的相关性

The expression of GPC⁃1,miRNA⁃9 and Caspase⁃3 in lung adenocarcinoma and its correlation with the condition and prognostic survival

目的探讨磷脂酰肌醇蛋白多糖-1(GPC-1)、微小RNA-9(miRNA-9)及天冬氨酸特异性半胱氨酸蛋白酶-3(Caspase-3)在肺腺癌中的表达及与病情、预后生存的相关性。方法 2015年8月至2017年8月本院收治的肺腺癌患者的临床资料进行整理、分析(76份肺腺癌组织标本及76份癌旁正常组织标本),采用免疫组化染色法对比肺腺癌组织及癌旁正常组织中GPC-1、miRNA-9及Caspase-3表达情况。结果肺腺癌组织中GPC-1阳性表达率、Caspase-3阴性表达率、miRNA-9高表达率明显高于癌旁正常组织(P<0.05)。GPC-1、miRNA-9及Caspase-3表达情况与肺腺癌患者年龄、性别、分化程度、肿瘤直径、血管侵犯无关(P>0.05);与TNM分期、淋巴结转移有关(P<0.05)。76例肺腺癌患者3年生存率为35.53%(49/76),GPC-1阳性表达、miRNA高表达、Caspase-3阴性表达的肺腺癌患者死亡率更高(P<0.05)。生存分析显示GPC-1(表达阴性)、miRNA-9(低表达)、Caspase-3(阳性表达)者生存期较长,Keplan-meier生存曲线明显不同(P<0.05)。临床分期(Ⅲ~Ⅵ期)、有淋巴结转移、GPC-1表达(阳性)、miRNA(高表达)及Caspase-3表达(阴性)为影响肺腺癌患者预后生存的独立危险因素(P<0.05)。结论GPC-1、miRNA、Caspase-3异常表达与肺腺癌患者病情进展密切相关,且不利于患者预后。

Objective To explore the expression of Glypicans⁃1(GPC⁃1),microRNA⁃9(miRNA⁃9)and Cysteinyl Aspartate⁃Specific Proteninases⁃3(Caspase⁃3)in lung adenocarcinoma and its correlation with the condition and prognostic survival.Methods The clinical data of patients with lung adenocarcinoma admitted to our hospital from August 2015 to August 2017 were sorted and analyzed(76 specimens of lung ade⁃nocarcinoma tissue and 76 specimens of normal tissue adjacent to cancer).Immunohistochemical staining was used to compare the expression of GPC⁃1,miRNA⁃9 and Caspase⁃3 in lung adenocarcinoma tissue and normal tissues adjacent to the cancer.Results The positive expression rate of GPC⁃1,the negative expression rate of Caspase⁃3 and high expression rate of miRNA⁃9 in lung adenocarcinoma tissue were significantly higher than those in normal tissues adjacent to cancer.The expression of GPC⁃1,miRNA⁃9 and Caspase⁃3 was not related to the age,gender,degree of differentiation,tumor diameter,and vascular invasion in patients with lung adeno⁃carcinoma(P>0.05),it was related to TNM staging and lymph node metastasis(P<0.05).The 3⁃year survival rate of 76 patients with lung adenocarcinoma was 35.53%(49/76),and the mortality of patients with lung ade⁃nocarcinoma and GPC⁃1 positive expression,high miRNA expression,and Caspase⁃3 negative expression was higher(P<0.05).Survival analysis showed that patients with GPC⁃1(expression negative),miRNA⁃9(low ex⁃pression),and Caspase⁃3(positive expression)had longer survival times,and Keplan⁃meier survival curves were significantly different(P<0.05).Clinical staging(stageⅢ~Ⅵ),lymph node metastasis,GPC⁃1 expres⁃sion(positive),miRNA(high expression)and Caspase⁃3 expression(negative)were independent risk factors affecting the prognosis of patients with lung adenocarcinoma(P<0.05).Conclusion Abnormal expressions of GPC⁃1,miRNA,and Caspase⁃3 are closely related to the disease progression of patients with lung adenocarci⁃noma and are not conducive to the prognosis of patients.