宫内暴露于高雄激素对小鼠胎盘营养转运体表达的影响

Effect of intrauterine exposure to high androgen on expression of placental nutritional transporter in mice

目的探讨宫内暴露于高雄激素对小鼠胎盘营养转运体表达的影响。方法选择2018年1-3月购置的6~8周龄ICR雌性小鼠,自然受精后6.5 d开始每天给予颈部皮下注射脱氢表雄酮(60 mg·kg^-1·d^-1)构建实验组,单纯玉米油注射构建对照组,第18.5天采用颈椎脱臼法处死小鼠后,收集胎鼠及胎盘标本,比较两组小鼠存活率、胎鼠体质量、胎盘质量、胎盘转运效率及目的基因mRNA表达水平。结果实验组与对照组胚胎总着床数、存活数差异均无统计学意义(P>0.05);实验组平均窝仔数、存活率低于对照组(P<0.05);实验组与对照组胎盘转运效率差异无统计学意义(P>0.05);实验组胎鼠体质量、胎盘质量均低于对照组(P<0.05);实时荧光定量PCR法检测两组胎盘的葡萄糖转运体mRNA表达水平,结果表明,与对照组相比,实验组胎盘Slc2a1、Slc2a2、Slc2a3、Slc2a9、Slc2a12 mRNA表达水平均出现不同程度下调(P<0.05);实验组Slc38a1、Slc38a2、Slc38a4、Slc43a2、Slc7a5、Slc7a8 mRNA表达水平下调(P<0.05)。结论宫内暴露于高雄激素中能降低胎盘质量与胎鼠体质量,能降低胎盘氨基酸转运体和葡萄糖转运体mRNA表达水平,提示宫内暴露于高雄激素会影响妊娠末期胎盘营养转运能力。

Objective To explore the effect of intrauterine exposure to high androgen on the expression of placental nutrition transporter in mice.Methods The 6-8 weeks ICR female mice purchased from January 2018 to March 2018 were selected,and the cervical subcutaneously injection of dehydroepiandrosterone(DHEA,60 mg·kg^-1·d^-1)on 6.5 d after natural fertilization was performed for constructing the experimental group.The control group was constructed by the injection of corn oil alone.After the mice were sacrificed at the neck on 18.5 d,the placental and fetal mouse placental specimens were collected to compare the survival rate,fetal rat body mass,placental mass,placental transport efficiency and target gene mRNA expression level between the two groups.Results The differences of total implantation and survival numbers between the experimental group and the control group were not statistically significant(P>0.05);the average litter size and survival rate of the experimental group were lower than that of the control group(P<0.05);the difference of placental transfer efficiency between the experimental group and the control group was not statistically significant(P>0.05);the fetal mouce mass and placenta mass in the experimental group were lower than those in the control group(P<0.05);the real-time quantitative PCR method was used to detect the expression level of glucose transporter mRNA in the two groups of placenta.The results showed that the expression levels of Slc2a1,Slc2a2,Slc2a3,Slc2a9,and Slc2a12 in the two group were down-regulated to varying degrees(P<0.05);in the experimental group,the expression levels of Slc38a1,Slc38a2,Slc38a4,Slc43a2,Slc7a5,and Slc7a8 mRNA were down-regulated(P<0.05).Conclusion Intrauterine exposure to high androgens can reduce placental mass and birth mouse mass,and can also reduce the mRNA expression levels of placental amino acid transporter and glucose transporter,suggesting that intrauterine exposure to high androgen will affect the ability of placental nutrient transport in late pregnancy.