特发性肺纤维化血清生物指示物的研究

Biomarkers in sera of idiopathic pulmonary fibrosis

目的:探讨Human Napsin-A(NASPA)、肺表面活性物质相关蛋白A(SP-A)及肺表面活性物质相关蛋白D(SP-D)、外泌体hsa-miR-21-5p、血清中hsa-miR-29b-3和hsa-miR-30c-5p在特发性肺纤维化中的变化及其临床意义。方法:选取2015年2月至2019年8月于河南省胸科医院就诊的50例特发性肺纤维化患者(特发性肺纤维化组)资料进行回顾性研究,将同期体检健康者20名作为健康对照组。对比2组患者在NASPA、SP-A、SP-D、hsa-miR-21-5p、hsa-miR-29b-3和hsa-miR-30c-5p表达水平等项目之间的差异。结果:2组一般资料比较差异无统计学意义(P>0.05),特发性肺纤维化组NASPA、SP-D、hsa-miR-21-5p的表达均要明显高于健康对照组(P<0.05),SP-A、hsa-miR-29b-3的表达均较健康对照组显著降低(P<0.05)。结论:血浆中SP-D检测能够作为特发性肺纤维化患者的潜在诊断指标;血浆中NASPA、外泌体hsa-miR-21-5p、血清中hsa-miR-29b-3检测可以作为特发性肺纤维化患者肺部疾病诊断的候选标志物,为临床诊断提供一定的指导作用。

Objective:To investigate the changes and clinical significance of Human Napsin-A(NAPSA),pulmonary surfactant protein A(SP-A),pulmonary surfactant protein D(SP-D),exosomes hsa-miR-21-5p,serum hsa-miR-29b-3 and hsa-miR-30c-5p in idiopathic pulmonary fibrosis.Methods:A total of 50 patients with idiopathic pulmonary fibrosis in Henan Chest Hospital from February 2015 to August 2019 were selected as the research objects(IPF group),and other 20 healtly persons in the same period were selected as control group.The expression levels of NASPA,SP-A,SP-D,hsa-miR-21-5p,hsa-miR-29b-3,and hsa-miR-30c-5p were compared between the two groups.Results:There was no significant difference in the general data between the two groups(P>0.05).The expressions of NASPA,SP-D and hsa-miR-21-5p in the IPF group were significantly higher than those in the healthy control group(P<0.05),and the expressions of SP-A and hsa-miR-29b-3 were significantly lower than those in the healthy control group(P<0.05).Conclusions:The detection of SP-D in plasma can be used as a potential diagnostic indicator for patients with idiopathic pulmonary fibrosis.Detection of NASPA in plasma,hsa-miR-21-5p in exosomes,and hsa-miR-29b-3 in serum can be used as candidate markers for the diagnosis of pulmonary diseases in patients with idiopathic pulmonary fibrosis,providing some guidance for clinical diagnosis.