基于网络药理学探讨丹参-红花药对治疗冠心病的作用机制

Study on the Mechanism of Herb Pair Radix Salviae Miltiorrhizae-Flos Carthami in Treating Coronary Heart Disease Based on the Network Pharmacology

目的:利用网络药理学的方法探讨丹参-红花药对治疗冠心病的作用机制。方法:运用TCMSP网络数据库对丹参-红花两味药主要有效成分及作用靶点进行筛选和挖掘,利用Cytoscape3.7.2软件,构建中药成分-靶点网络图;以OMIM在线分析平台挖掘冠心病的相关作用靶点,基于STRING数据库构建丹参-红花治疗CHD的蛋白质-蛋白质相互作用(PPI)网络图,然后进行拓扑分析,筛选出丹参-红花治疗冠心病的核心靶点。利用Metascape数据库对药物-疾病交集靶点进行生物通路及富集分析。结果:丹参-红花成分-靶点网络图包含87个有效成分,相对应靶点1 693个;核心靶点涉及TNF、白细胞介素-1B(IL-1B)、髓过氧化物酶(MPO)、血管细胞黏附蛋白1(VCAM1)、血管内皮生长因子A(VEGFA)、基质金属蛋白酶3(MMP3)等。结论:丹参-红花治疗冠心病主要靶点参与与流体剪切应力与动脉粥样硬化、活性氧代谢过程、DNA结合转录因子活性的正调控、蛋白质复合物装配的正调控、细胞活化的调节等相关,其中聚集在流体剪切应力与动脉粥样硬化的靶点最多。

Objective:To explore and analyze the mechanism of Radix Salviae Miltiorrhizae-Flos Carthami herb pair on the treating coronary heart disease through network pharmacology.Methods:The TCMSP network database was used to screen and mine the main effective components and targets of Danshen-Honghua,and the Cytoscape3.7.2 software was used to construct the network diagram of traditional Chinese medicine components and targets.The OMIM online analysis platform was used to mine the relevant targets of coronary heart disease.Based on STRING database,the protein-protein interaction(PPI)network diagram(PPI)of Danshen-Honghua for CHD treatment was constructed.Then topological analysis was performed to screen out the core targets of Danshen-Honghua for CHD treatment.Metascape database was used to analyze the biological pathway and enrichment of drug-disease intersection targets.Results:The network diagram of Danshen-Honghua and target contains 87 active components and 1693 corresponding targets.The core targets include TNF,1L1B,MPO,VCAM1,VEGFR,MMP3,etc.Conclusion:It can be seen that the main target participation of Danshen-Honghua in treating coronary heart disease is related to fluid shear stress and atherosclerosis,active oxygen metabolism process,positive regulation of DNA binding transcription factor activity,positive regulation of protein complex assembly,regulation of cell activation,etc.Among them,the largest amount of targets were concentrated in fluid shear stress and atherosclerosis,which further illustrated that Danshen-Honghua had more pathways and more complicated mechanisms in treating coronary heart disease,and also suggested that this biological process might be an important direction for future research on Danshen-Honghua in treating CHD.