LncRNA OSER1-AS1通过调控miR-433-3p影响宫颈癌细胞增殖、迁移及侵袭

LncRNA OSER1-AS1 Affects the Proliferation,Migration and Invasion of Cervical Cancer Cells by Regulating miR-433-3p

该研究旨在探讨lncRNA OSER1-AS1对宫颈癌细胞增殖、迁移、侵袭的影响及其对miR-433-3p的调控作用。利用Western blot和qRT-PCR方法检测宫颈癌细胞中OSER1-AS1、miR-433-3p的表达水平,发现宫颈癌细胞中OSER1-AS1的表达水平显著升高(P<0.05),而miR-433-3p的表达水平显著降低(P<0.05);将si-NC、si-OSER1-AS1、miR-NC、miR-433-3p、si-OSER1-AS1+anti-miR-NC、si-OSER1-AS1+anti-miR-433-3p转染至SiHa细胞,进一步应用CCK-8法与平板克隆实验检测细胞增殖能力,发现转染si-OSER1-AS1或miR-433-3p mimics后,细胞活力显著降低(P<0.05),克隆形成数、迁移及侵袭细胞数显著减少(P<0.05);而转染si-OSER1-AS1+anti-miR-433-3p后,细胞活力显著升高(P<0.05),且克隆形成数、迁移及侵袭细胞数显著增多(P<0.05);最后用双荧光素酶报告实验检测OSER1-AS1、miR-433-3p的靶向关系,发现OSER1-AS1能够竞争性地结合miR-433-3p。结果说明,OSER1-AS1可负向调控miR-433-3p的表达进而调控细胞增殖、迁移及侵袭。提示干扰OSER1-AS1表达可通过上调miR-433-3p的表达从而抑制宫颈癌细胞的增殖、迁移及侵袭。

This study is aimed to explore the effect of lncRNA OSER1-AS1 on the proliferation,migration and invasion of cervical cancer cells and its regulation on miR-433-3p.Western blot and qRT-PCR were used to detect the expression levels of OSER1-AS1 and miR-433-3p in cervical cancer cells,finding that the expression level of OSER1-AS1 in cervical cancer cells was significantly increased(P<0.05),while the expression level of miR-433-3p was significantly reduced(P<0.05).si-NC,si-OSER1-AS1,miR-NC,miR-433-3p,si-OSER1-AS1+anti-miR-NC,and si-OSER1-AS1+anti-miR-433-3p were transfected into SiHa cells.Furthermore,CCK-8 method and plate cloning experiment were used to detect cell proliferation ability,finding that transfection of si-OSER1-AS1 or miR-433-3p mimics significantly reduced cell viability(P<0.05),and the number of clone formation,migration and invasion cells were significantly reduced(P<0.05).However,when transfected with si-OSER1-AS1+anti-miR-433-3p,cell viability was significantly increased(P<0.05),and the number of clone formation,migration and invasion cells were significantly increased(P<0.05).Finally,the dual luciferase reporter experiment was used to detect the targeting relationship of OSER1-AS1 and miR-433-3p,finding that OSER1-AS1 could competitively bind to miR-433-3p.The results indicated that OSER1-AS1 could negatively regulate the expression of miR-433-3p and mediate cell proliferation,migration and invasion.It was suggested that interference with OSER1-AS1 expression could inhibit the proliferation,migration and invasion of cervical cancer cells by up-regulating the expression of miR-433-3p.