玉米芯高温热解提取戊聚糖工艺优化

Optimization of Extracting Pentosan from Corncob by High Temperature Pyrolysis

以玉米芯为原料,采用高温热解法提取戊聚糖,在单因素试验基础上,采用正交试验法对提取工艺参数进行优化,利用高效液相色谱法对戊聚糖提取液进行组分分析。结果表明:最佳提取工艺条件为固液比1∶15(g/mL),热解温度180℃,热解时间60 min,在此条件下提取率为25.84%。色谱图结果显示:提取液中含有大量的戊聚糖、木二糖、木三糖、木四糖、木五糖和木六糖,为后续酶解工艺制备低聚木糖提供原料。

The corncob was used as raw material to extract pentosan by high temperature pyrolysis.Based on the screening of single-factor experiment design,the extraction process parameters were optimized by orthogonal test method.The prepared pentosan extract was subjected to component analysis by high performance liquid chromatography.The results showed that the optimum extraction conditions were 1∶15(g/mL)ratio of material to liquid,180℃pyrolysis temperature and 60 min pyrolysis time.Under this condition,the extraction rate was 25.84%.The chromatogram results showed that the extract contains a largeamount of pentosan,xylobiose,xylotriose,xylotetraose,xylopentaose and xylohexaose req-uired to provide raw materials for the preparation of xylooligosaccharides by subsequent enzymatic hydrolysis.