皮肤鳞状细胞癌Nrf2和Keap1的表达分析

The Expression of Nrf2 and Keap1 in the Skin Squamous Cell Carcinoma

目的:通过研究核因子E2相关因子2(Nrf2)和Keleh样环氧氯丙烷相关蛋白1(Keap1)在皮肤鳞状细胞癌中的表达情况,探讨Nrf2和Keap1在皮肤鳞状细胞癌的发生发展过程的作用机制。方法:选取手术切除人颜面部病理学确诊鳞状细胞癌标本(试验组)及癌旁正常组织病理学标本(对照组),进行固定切片行免疫组织化学检查,观察皮肤鳞状细胞癌及正常皮肤中Nrf2和Keap1阳性表达率。结果:试验组Keap1及Nrf2蛋白阳性表达率均低于对照组,差异均有统计学意义(P<0.05)。结论:皮肤鳞状细胞中Nrf2及Keap1低表达或无表达,可能与皮肤鳞状细胞癌的发生有关。

Objective:By studying the expression of nuclear factor E2-related factor 2(Nrf2)and Keleh-like epichlorohydrin-related protein 1(Keap1)in skin squamous cell carcinoma,the mechanism of Nrf2,Keap1 and its occurrence and development in skin squamous cell carcinoma was discussed.Method:Specimens of SCC confirmed by surgical excision of human facial pathology(experimental group)and adjacent normal histopathological specimens(control group)were selected,immunohistochemical examination was performed on fixed sections to observe the positive expression rates of Nrf2 and Keap1 in squamous cell carcinoma of the skin and normal skin.Result:The positive expression rates of Keap1 and Nrf2 proteins in the experimental group were lower than those in the control group,the differences were statistically significant(P<0.05).Conclusion:Nrf2 and Keap1 are low or no expressed in squamous cell skin,may be related to the occurrence of a squamous cell carcinoma of the skin.