落地生根冻干粉对人癌细胞增殖和细胞凋亡的影响

The effect of Bryophyllumpinnatum freeze-dried powder on human cancer cell lines proliferation

为初步研究落地生根冻干粉(BPFP)对人癌细胞增殖的影响,应用CCK-8实验检测不同质量浓度BPFP对人肝癌HepG2细胞、人脑胶质瘤U87细胞和人前列腺癌DU145细胞增殖的影响;用Hoechst凋亡试剂盒检测不同质量浓度BPFP对细胞凋亡的影响.实验结果显示:BPFP对HepG2、U87和DU145细胞生长均有一定的抑制作用;BPFP作用48 h对HepG2细胞增殖抑制作用明显,抑制率在82%以上,其抑制程度与时间呈依赖性,但与剂量不呈依赖性;质量浓度为150μg/mL以上作用48 h对U87细胞增殖抑制明显,增殖抑制率大于80%;质量浓度为250μg/mL作用48 h对DU145增殖抑制作用最强,达到100%.BPFP作用24 h对HepG2、U87和DU145细胞凋亡均有一定的促进作用.实验结果提示,BPFP对HepG2、U87和DU145细胞的增殖有抑制作用,可能与BPFP能促进细胞凋亡有关.

In order to study the effect of Bryophyllumpinnatum freeze-dried powder(BPFP)on the proliferation of human cancer cell lines,CCK-8 assay was used to detect the effect of different concentrations of BPFP on the anti-proliferation activity of human cancer cell lines HepG2(liver)U87(glioma),DU145(prostate),and Hoechst apoptosis kit was used to detect the effect of BPFP on apoptosis.The experimental results showed that the growth of HepG2,U87 and DU145 cells were inhibited by BPFP,and the inhibition rate on HepG2 was more than 82%after 48 h treatment.The BPFP inhibited the proliferation of HepG2 with a dose but not time dependent manner.The inhibition rate on U87 was more than 80%after 48 h treatment when the concentration was above 150μg/mL after 48 h treatment.At the concentration of 250μg/mL for 48 h,the inhibition of DU145 proliferation was the strongest,reaching 100%.The BPFP can induce the apoptosis of HepG2,U87 and DU145 cells to a certain extent at 24 h.The experimental results demonstrated that BPFP can inhibit the proliferation of HepG2,U87 and DU145 cell lines,which may be related to the fact that BPFP can induce cell apoptosis.