毛梗豨莶乙酸乙酯部位化学成分及生物活性研究

Chemical components and bioactivity from the ethyl acetate of Siegesbeckia glabresce

毛梗豨莶草(Siegesbeckia glabresce)的乙酸乙酯萃取部位具有显著的抑制细胞坏死性凋亡(necroptosis)的活性。为明确毛梗豨莶草的活性成分,该研究采用正相硅胶柱色谱谱(二氯甲烷∶甲醇20∶1~0∶1)、反相ODS柱色谱(30%~100%甲醇)、Sephadex LH-20柱色谱、重结晶等方法对毛梗豨莶草乙酸乙酯萃取部位进行了化学成分分离和纯化,并根据理化性质和波谱数据进行了结构鉴定。结果表明:从毛梗豨莶乙酸乙酯部位分离并鉴定了9个化合物,分别为3,7-二甲氧基槲皮素(1)、芹菜素(2)、奥卡宁(3)、okanin-4’-O-β-D-6″-trans-p-coumaroylglucoside(4)、1H-Indole-3-carbaldehyde(5)、对羟基苯甲醛(6)、3,5-二甲氧基-4-羟基苯甲醛(7)、3,4-divanillyltetrahydrofuran(8)、buddlenol D(9)。除化合物1和化合物6外,其余7个化合物均为首次从豨莶属植物中分离得到。

The ethyl acetate extracts of Siegesbeckia glabrescens had significant physiological activity to inhibit necroptosis.In order to identify the active components and find the target compounds,the ethyl acetate extracts of S.glabresce were isolated and studied systemically in chemistry.The compounds were isolated and purified by chromatography on silica gel,ODS,and Sephadex LH-20 columns,and their structures were determined according to physicochemical properties and spectral analyses.Nine compounds were isolated from the ethyl acetate of S.glabresce and identified as 3,7-dimethoxyquencetin(1),apigenin(2),okanin(3),okanin-4’-O-β-D-6″-trans-p-coumaroylglucoside(4),1 H-indole-3-carbaldehyde(5),p-hydroxybenzaldehyde(6),3,5-dimethoxy-4-hydroxybenzaldehyd(7),3,4-divanillyltetrahydrofuran(8),buddlenol D(9).Compounds 2-5,7-9 were isolated in this herbal plant for the first time.