南沙石珊瑚来源真菌 Aspergillus terreus SCAU139的聚酮类代谢产物研究

Research on Polyketides of Secondary Metabolites from a Stony Coral associated Fungal Strain Aspergillus terreus SCAU139 Collected from the Nansha Islands

为了从海洋来源真菌的次生代谢产物中寻找抗植物病原真菌药物先导结构,采用PCR扩增技术对一株南沙石珊瑚来源真菌进行种属鉴定,运用柱色谱分离方法和波谱分析手段分离鉴定该菌株的次级代谢产物,并结合滤纸片扩散法测试单体化合物的抗菌活性。菌株鉴定为Aspergillus terreus,从其代谢产物中分离鉴定出7个已知化合物,分别为4-hydroxy-3-(3-methyl-2-buten-1-yl)-benzaldehyde(1)、terrein(2)、4-hydroxybenzaldehyde(3),monacolin K(4)、butyrolactone I(5)、pernolide D(6)、hydroxysulochrin(7),以上单体化合物对苹果黑点致病菌Alternaria alternate和菠萝黑心致病菌Curvularia australiensis的生长无明显抑制效果。石珊瑚来源真菌的次级代谢产物含有多种结构类型的聚酮类化合物。

The secondary metabolites of a stony coral-associated fungal strain Aspergillus terreus SCAU139 collected from Nansha Islands were investigated by methods of column chromatographic isolation and spectroscopic analysis.In order to find the leading structure of anti-phytopathogenic fungus drugs from the secondary metabolites of marine-derived fungi,PCR amplification technology was used to identify the species of a fungus from Nansha stony coral.The column chromatography and spectral analysis methods were used to separate and identify the secondary metabolites of the strains and to test the antibacterial activity of the monomer compounds combined with the filter paper diffusion method.The strain was identified as Aspergillus terreus,and 7 known compounds were isolated and identified from its metabolites,namely 4-hydroxy-3-(3-methyl-2-buten-1-yl)-benzaldehyde (1),terrein (2),4-hydroxybenzaldehyde (3),monacolin K (4),butyrolactone I (5),pernolide D (6),hydroxysulochrin (7).The above monomer compounds have no obvious inhibitory effect on the growth of Alternaria alternate and Curvularia australiensis.The secondary metabolites of fungi derived from stony corals contain polyketides of various structural types.