深海真菌Trichobotrys effuse DFFSCS021的次级代谢产物研究

Studies on the Secondary Metabolites of Deep sea derived Fungus Trichobotrys effuse DFFSCS021

通过多种现代化波谱手段,对深海真菌Trichobotrys effuse DFFSCS021的次级代谢产物进行挖掘。采用硅胶柱层析、Sephadex LH-20凝胶柱层析和高效液相色谱等分离技术,对该菌株发酵产物中的化学成分进行研究,并运用核磁共振(NMR)、质谱(MS)和文献比对鉴定其化学结构。结果显示:从该菌株的发酵产物中共分离得到5个单体化合物,分别为1,3-dimethyluracil(1)、[2E,7E]-9-oxo-2,7-decadienoic acid(2)、periconianones H(3)、botryosphaeridione(4)和2-hydroxy-(4-octyloxy)benzophenone(5),化合物1-5均是首次从该菌株中分离得到。化合物3对5株肿瘤细胞株HepG-2、Eca109、KG-1a、PC-3和Hep2均未显示出细胞毒活性,仅对Hela显示弱的抑制活性。实验结果不仅丰富了该菌属的化学成分类型,而且也增强了深海真菌的研究价值。

Through a variety of modern spectrum methods,the secondary metabolites of a deep-sea-derived fungus Trichobotrys effuse DFFSCS021 were excavated.The chemical components of the fermentation products of this strain were isolated and purified by silica gel,Sephadex LH-20,and semi-preparative reverse-phase HPLC (C18) and other separation techniques.Its chemical structure was identified by nuclear magnetic resonance (NMR),mass spectrometry (MS) and literature comparison.The results showed that a total of 5 monomer compounds were isolated from the fermentation product of the strain and their structures were identified as 1,3-dimethyluracil (1),[2E,7E]-9-oxo-2,7-decadienoic acid (2),periconianones H (3),botryosphaeridione (4) and 2-hydroxy-(4-octyloxy)benzophenone (5).The compounds 1-5 were all isolated from this strain for the first time.The experimental results not only enriched the types of chemical components of the genus,but also improved the research value of the deep-sea-derived fungus.