吡菲尼酮对UUO大鼠肾纤维化组织中TGF-β1/smad3通路表达的影响

Effect of pifenidone on TGF-1/smad3 pathway expression in renal fibrosis in UUO rats

目的:探讨吡菲尼酮对UUO大鼠肾组织中TGF-β1/smad3通路表达的影响及作用。方法:将72只Wistar雄性大鼠随机分为3组:sham组、UUO组以0.9%NaCl 2mL灌胃,治疗组给予吡菲尼酮500mg/kg溶解后灌胃,各组大鼠于术后第3,7,14天分别处死8只。结果:与Sham组相比,UUO模型组肾小管间质损伤和纤维化程度明显,TGF-β1及smad3明显表达增多;且随着梗阻时间延长,UUO模型组大鼠的TGF-β1及smad3表达逐渐增加。与模型组比较治疗组大鼠肾间质组织中TGF-β1及smad3的表达水平显著降低。结论:吡菲尼酮能够通过抑制TGF-β1/smad3信号通路产生延缓肾间质纤维化的作用,可能为肾间质纤维化的临床治疗提供新的论证。

Objective:To investigate the effect of pifenidone on the expression of TGF-1/smad3 pathway in UUO renal tissue.Methods:Seventy-two wistar male rats were randomly divided into 3 groups:Sham group,UUO group and treatment group,with 24 rats in each group.In the UUO group,2 mL 0.9%NaCl was given by gavage,and in the treatment group,500 mg/kg of pifenidone was given by gavage,and 8 rats in each group were sacrificed on the 3rd,7th and 14th days after surgery.Results:Compared with Sham group,renal tubular interstitial damage and fibrosis degree were obvious in UUO model group,TGF-1 and smad3 were significantly increased.The expression of TGF-1 and smad3 in the UUO model group increased gradually with the extension of obstruction time.The expression levels of TGF-1 and smad3 in renal interstitial tissues of rats in the treatment group were significantly decreased compared with that in the UUO model group.Conclusion:Pifenidone can delay renal interstitial fibrosis by inhibiting TGF-1/smad3 signaling pathway,which may provide a new demonstration for the clinical treatment of renal interstitial fibrosis.