蔗糖对84K杨树组培继代苗生长及生理特性的影响

Effects of Sucrose on Growth and Physiological Characteristics in vitro Plantlets of 84K Poplar

碳源对组培苗的生长和品质具有重要影响,本实验研究了不同蔗糖浓度(10、20、30和40 g·L^-1)对84K杨树组培继代苗的生长及生理特性的影响。研究结果表明,随着培养时间的增长,30 g·L^-1的蔗糖浓度最有利于84K杨树组培继代苗的生长,其干重是10 g·L^-1蔗糖处理的1.5倍。其次,在较高的蔗糖浓度处理中,随着培养时间的增加,84K杨树组培继代苗具有较高的叶绿素含量、Rubisco酶活性及净光合速率,其中30 g·L^-1蔗糖处理的84K杨组培苗净光合速率最大,为3.617μmol·m^-2·s^-1。同时,高浓度蔗糖处理下的84K杨树组培继代苗有较高含量的可溶性糖,但其体内淀粉含量却无明显变化。

Carbon source has an important effect on the growth and quality in vitro plantlets.The effects of different sucrose concentrations(10,20,30 and 40 g·L^-1)on the growth and physiological characteristics in vitro plantlets of 84K poplar were studied.The results showed that with the 30 g·L^-1 sucrose was the most favorable for the growth in vitro plantlets of 84K poplar with the culture period increased,and the dry weight was 1.5 times that of 10 g·L^-1 sucrose concentration treatment.Secondly,in the treatment of higher sucrose concentration,with the culture period increased,the in vitro plantlets of 84K poplar had higher chlorophyll content,Rubisco enzyme activity and net photosynthetic rate,of which 30 g·L^-1 sucrose treatment in vitro plantlets of 84K poplar had the highest net photosynthetic rate,which was 3.617μmol·m^-2·s^-1.The in vitro plantlets of 84K poplar treated with high concentration of sucrose had higher soluble sugar content,but no significant change in starch content.To sum up,sucrose concentration has an important influence on the growth and physiological characteristics in vitro plantlets of 84K poplar.Higher sucrose concentration was conducive to promoting the growth in vitro plantlets of 84K poplar.