毛果杨GATA基因家族全基因组水平鉴定及表达分析

Genome-wide Identification and Expression Analysis GATA Family of Populus trichocarpa

GATA转录因子基因家族在植物生长发育、细胞分化以及响应环境变化中具有重要作用。然而,目前在木本植物中尚无该基因家族全基因组水平的分析报导。本项研究从基因组水平对毛果杨GATA家族成员的数量、基因结构、染色体定位、系统进化、编码蛋白的理化特征和保守基序等信息进行了系统分析,结果表明,毛果杨GATA家族包含39个基因,共分布于15条染色体上,其中5号染色体上含有6个基因,9号、13号和19号染色体含有基因数量为1,其余染色上无基因分布。该家族各基因的结构与编码蛋白的基本特性均存在一定异性,可分成4个亚族。qRT-PCR研究表明,GATA家族各基因在不同发育阶段的茎部表达量存在明显差异,且盐胁迫对各基因的表达特性影响显著。以上结果表明,毛果杨GATA家族基因在复制后,基因的结构与功能产生了明显分化,其中部分基因在毛果杨次生生长与盐胁迫响应中可能具有重要作用。本项研究为全面解析毛果杨GATA家族各成员在其生长发育与盐胁迫响应中的生物学功能奠定了基础。

The GATA transcription factor gene family played an important role in plant growth and development,cell differentiation,and response to environmental changes.However,no genome-wide analysis of this gene family has been reported in woody plants so far.We systematically analyzed the number,gene structure,chromosome positioning,phylogenetic evolution,physicochemical characteristics and conserved motifs of the GATA family members from the genome level respectively.The results showed that the GATA family contains 39 genes distributed on 15 chromosomes,and 6 genes distributed on chromosome 5,and only 1 gene distributed on chromosomes 9,13 or 19 respectively.The rest chromosomes had no gene distributed on.The genes structure in this family and the basic characteristics encoded protein were somewhat heterogeneous,and could be divided into 4 subfamilies.By the qRT-PCR,there were significant differences in the expression levels of the genes of the GATA family at different developmental stages,and salt stress significantly affected the expression characteristics of the genes.The above results indicated that the structure and function of the genes were significantly differentiated after the replication of GATA family genes,and some of these genes might play an important role in the secondary growth of Populus trichocarpa and the response to salt stress.This study laid the foundation for a comprehensive analysis of the biological functions of the members of the GATA family of P.trichocarpa in their growth and development and response to salt stress.