血清AFP阴性肝癌中EGFR的表达与临床及预后的关系

Expression of EGFR in serum AFP negative hepatocellular carcinoma and its relationship with clinicopathological and prognosis

目的探讨血清AFP阴性的肝细胞肝癌癌组织表皮生长因子受体(epidermal growth factor receptor,EGFR)的表达与临床病理特征及预后的关系。方法用BenchMarkXT全自动免疫组化机检测癌组织ECFR表达情况,x检验分析与临床病理参数间的关系,Kaplan-Meier法及Cox风险模型进行生存分析。结果56例血清AFP阴性肝癌患者EGFR阳性率78.6%,肿瘤分级3级的EGFR阳性率高于1级.2级患者,有饮酒史患者的EGFR阳性率高于无饮酒患者,差别均有统计学意义(P=0.003,P=0.005),不同性别年龄组、脉管癌栓、肿瘤大小、血清HBsAg有否阳性、周围肝组织有否肝硬化及有无吸烟史中,EGFR表达率差别均无统计学意义;Kaplan-Meier生存分析发现EGFR阳性表达患者生存率高于阴性患者,差异有统计学意义(P=0.045),Cox多因素分析发现癌组织EGFR的表达及术后化疗是预测肿瘤预后的独立因素(P<0.05)。结论EGFR的阳性表达与肿瘤分级及饮酒史正相关,EGFR的表达在--定程度上影响血清AFP阴性的肝细胞肝癌的发生发展及预后。

Objective To investigate the expression of epidermal growth factor receptor(EGFR)in hepatocellular carcinoma(HCC)with negative serum AFP and its relationship with elinicopathological features and prognosis.Methods The expression of EG-FR in HCC with serum AFP negative was detected by BenchMark XT of Roche.The relationship between clinical pathology and ECFR was analysised by chi square.The survival analysis application of Kaplan Meier method,and by using the Cox proportional hazards model analysis of the independent risk factors for the prognosis.Results Among the 56 patients with serum AFP negative hepatocellu-lar carcinoma,the positive rate of EGFR was 78.6%.The positive ratle of EGFR in diferent tumor grades was significantly different(P=0.003).Bot Kaplan-Meier test and Cox regression model showed the expression of EGFR and postoperative chenotherapy were in-dependent prognostic factors ofHCC with serum AFP negative.Conclusion The positive expression of EGFR is positively correlated with tumor grade and drinking history.The expression of EGFR may influence the occurence,development and prognosis of HCC with serum AFP negative.