丙硫菌唑悬浮剂中有效成分反相高效液相色谱分析

Analysis of Prothioconazole in the SC Formulation by RP-HPLC

[目的]建立一种利用反相高效液相色谱测定丙硫菌唑悬浮剂中有效成分的分析方法。[方法]采用反相C18色谱柱,以甲醇-0.1%磷酸水溶液(体积比80∶20)为流动相,柱温为25℃,流速1.0 m L/min,DAD检测器设定检测波长为256 nm进行高效液相色谱分离。[结果]在上述分析条件下,丙硫菌唑在0.050~0.800 g/L质量浓度范围内线性关系良好,相关系数为0.9999,28%丙硫菌唑·多菌灵悬浮剂和30%丙硫菌唑可分散油悬浮剂的标准偏差分别为0.036、0.281,变异系数分别为1.194%、0.976%,平均回收率为100.1%、100.5%、100.7%和100.0%、99.8%、100.2%。[结论]该分析方法操作简便,分离效果好,精密度和准确度高,可用于丙硫菌唑悬浮剂中丙硫菌唑的定量分析。

[Aims] This study aims to establisha a detection method for determination of prothioconazole in its SC formulation by RP-HPLC. [Methods] The RP-HPLC separation was carried on a C18 column at 25 ℃, mobile phase of methanol-0.1% phosphoric acid(80:20, by vol) was used with a flowing rate of 1.0 mL/min, diode array detector was set at 256 nm. [Results] Under the optimized conditions, prothioconazole determination could be well donein the range of0.050-0.800 g/L(R2= 0.9999), the standard deviations of two SCs were 0.036 and 0.281, the variation coefficients were1.194 and 0.976% respectively. The average recovery were 100.1, 100.5, 100.7% and 100.0, 99.8, 100.2%.[Conclusions] The method is simple, highly resolutive, precise and accurate. It can be used for quantitative analysis of prothioconazole in its formulation.