发酵罐酸洗钝化对百日咳杆菌生长和产毒的影响

Effects of fermenter pickling and passivation on growth of Bordetella pertussis and expression of toxin

目的:探讨发酵罐酸洗钝化对百日咳杆菌生长和产毒的影响。方法:定期对百日咳发酵罐进行酸洗钝化处理,去除其表面附着的代谢螯合物。通过检测后续培养收获物菌浓度(550 nm吸光度值)、一次盐析沉淀量、发酵产物中百日咳毒素(pertussis toxin,PT)和丝状血凝素(filamentous haemagglutinin,FHA)含量,比较分析酸洗钝化对发酵培养和产毒的影响。结果:对处理前后各连续10批相关指标进行比较,处理后菌体550 nm吸光度均值由4.663提高至5.490,一次盐析沉淀量均值由4.84 g/L提高至5.90 g/L,PT平均含量达到3.3μg/ml,FHA平均含量达到7.6μg/ml,处理前后各连续10批菌体浓度和一次盐析沉淀量数据采用t检验计算出P值分别为0.03和0.05,差异均有统计学意义。结论:定期对发酵罐进行酸洗钝化可有效改善百日咳杆菌发酵及产毒水平。

Objective To investigate the effects of fermenter pickling and passivation on the growth of Bordetella pertussis and the toxin expression.Methods The metabolic chelates attached to the surface of fermenter were eliminated by pickling and passivation.Then the subsequent bacterial concentration of the cultivation,the weight of the first salting-out precipitation,the pertussis toxin(PT)/filamentous haemagglutinin(FHA)concentrations of the culture were detected to estimated the effect of the fermenter pickling and passivation.Results Ten successive results showed that the mean value of bacterial concentration increased from 4.663 to 5.490,and the mean value of first salting-out precipitation increased from 4.84 g/L to 5.90 g/L.The average PT content reached 3.3μg/ml and FHA reached 7.6μg/ml,The data of 10 successive results of bacterial cell concentration and first salting-out precipitation were calculated by t test with P values of 0.03 and 0.05,respectively,and the differences in the data were statistically significant.Conclusion Fermenter pickling and passivation is an effective way to improve the growth of Bordetella pertussis and the toxin expression.