负载生育酚的玉米醇溶蛋白纳米颗粒的构建及性质表征

Construction and Characterization of Zein Nanoparticles Loaded with Tocopherol

采用反溶剂法制备负载生育酚(TOC)的玉米醇溶蛋白(zein)-阿拉伯胶的复合纳米颗粒。探究不同zein∶AG比例,不同搅拌速度对zein-AG纳米颗粒稳定性的影响;不同pH值,不同盐离子浓度对负载TOC的zein-AG纳米颗粒稳定性的影响。试验结果表明:当zein∶AG质量比1∶1.5,搅拌速度800 r/min时所得纳米颗粒稳定性较好,粒径125.4 nm,多分散指数(PDI)0.19,电位-32.8 mV;当pH=3~9,盐离子浓度小于20 mmol/L时,zein-AG纳米颗粒相对稳定,表明zein和AG可有效结合,产生电荷屏蔽效应以抵御一定浓度的盐离子;负载TOC的zein纳米颗粒在结合AG后的稳定性显著提高,比负载TOC前对DPPH、ABTS和超氧阴离子都有更高的清除能力;zein/AG-TOC纳米颗粒能够在胃肠道模拟试验中实现缓释。

In this paper,zein-arabic gum(AG)nanoparticles loaded with tocopherol(TOC)were prepared by antisolvent method.The effects of different zein∶AG ratios and the stirring speeds on the stability of zein-AG nanoparticles were investigated,and the effects of different pH and the different concentrations of salt were also investigated.The experimental results showed that the nanoparticles with zein∶GA ratio of 1∶1.5 and the stirring speed of 200 r/min were stable,and the particle size was 125.4 nm,the polydispersity index(PDI)was 0.19 and the zeta-potential was-32.8 mV.When the pH=3~9,and the concentration of salt ion was less than 20 mmol/L,the zein-AG nanoparticles were stable which proved that zein and AG were bound effectively and had a certain charge shielding effect.The stability of zein-AG nanoparticles loaded with TOC was significantly improved,and the removal capacity of DPPH,ABTS and superoxide anions were higher than that of zein-AG nanoparticles.Moreover,zein/AG-TOC nanoparticles could achieve slow-release in gastrointestinal simulation experiments.