摘要
目的探讨代谢型谷氨酸受体5(mGluR5)对白色脂肪细胞棕色化的调控作用。方法Western blot检测mGluR5是否在白色及棕色脂肪组织中表达。用mGluR5拮抗剂处理分化的小鼠白色脂肪前体细胞系3T3L1,BODIPY荧光染色观察脂肪细胞脂滴形态,实时荧光定量聚合酶链反应(RT-qPCR)检测棕色脂肪细胞特异性基因及线粒体特异性基因的表达。通过细胞耗氧量(OCR)检测mGluR5拮抗剂处理后脂肪细胞呼吸能力及通过甘油释放试验检测脂肪分解能力。结果mGluR5在白色脂肪组织及棕色脂肪组织中均表达且在白色脂肪组织中表达更高(P<0.05)。抑制mGluR5可调节脂肪细胞脂滴形态,对棕色脂肪特异性基因及线粒体相关基因的表达、细胞呼吸、脂肪分解能力有促进的趋势,但变化差异无统计学意义。结论抑制mGluR5可促进白色脂肪细胞棕色化改变。
Objective To investigate whether mGluR5 could regulate white adipocytes browning.Methods Western blot was used to test the expression of mGluR5 in white and brown adipose tissue.Lipid droplets morphology was microscoped after BODIPY staining.Brown adipocytes specific genes and mitochondrial related genes were analyzed by RT-qPCR in mGluR5 antagonists treated 3T3L1 cells.OCR experiments and free glycerol release assay were carried out in 3T3L1 cells after incubation with mGluR5 antagonists.Results mGluR5 expressed in both white and brown adipose tissues.The expression in white fat was higher than that in brown fat(P<0.05).Lipid droplets morphology in adipocytes changed after treatment with mGluR5 antagonists.mGluR5 antagonists promoted the expression of browning genes and mitochondrial related genes,increased mitochondrial respiration rate and promotes lipolysis in 3T3L1 cells.Conclusions Inhibition of mGluR5 may promote white adipocyte browning.
作者
罗燮
曾粒
袁磊
LUO Xie;ZENG Li;YUAN Lei(Department of Endocrinology,the Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China;Department of Nephrology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China)
出处
《基础医学与临床》
2021年第1期38-43,共6页
Basic and Clinical Medicine
基金
国家自然科学基金青年科学基金(81800771)。
