葫芦藤外植体的优化消毒方法研究

Study on the Optimal Disinfection Method of Gourd Vine Explants

目的:优化葫芦藤外植体消毒的工艺条件.方法:采用响应面分析法中的中心组合设计方法(central composite design,CCD),建立以75%乙醇消毒时间、次氯酸钠浓度和次氯酸钠消毒时间对葫芦藤消毒效果影响的二次回归模型.结果:葫芦藤外植体的最佳消毒工艺条件为75%乙醇浸泡消毒时间8 s,次氯酸钠溶液浓度2%,次氯酸钠溶液浸泡消毒时间12 min,葫芦藤外植体存活率最高(56%),实验操作总时间约40 min.结论:该回归模型有极显著性,可以用来合理预测葫芦藤外植体的消毒效果.

The objective of the study is optimization of the process conditions for disinfection of gourd vine explants.The study takes a quadratic regression model with the effect of 75%alcohol disinfection time,Sodium hypochlorite concentration and disinfection time of sodium hypochlorite on disinfection effect of gourd vine explants was established using the central composite design(CCD)in response surface analysis.Results show that the optimum disinfection process conditions of gourd vine explants were 75%alcohol immersion disinfection time 8 s,sodium hypochlorite solution concentration 2%,sodium hypochlorite solution immersion disinfection time 12 min,the highest survival rate(56%),the total experimental operation time about 40 min.The conclusion is that the regression model is significant,and can be used to reasonably predict the disinfection effect of gourd vine explants.