170株肠道门诊来源副溶血性弧菌特征分析

目的分析肠道门诊患者分离副溶血性弧菌(VP)的毒力基因、血清分型及药敏特征,为当地食源性疾病的防控提供科学有力依据。方法对2015~2019年间肠道门诊就诊患者粪便中分离所得的170株VP进行毒力基因(tdh、trh、tlh)检测,O、K血清分型以及20种临床常用抗菌药物试验。结果170株VP均携带tdh、th基因,未检测到携带trh;血清型以O3:K6检出最多;临床常用的20种抗菌药中,对AMP、KZ、PRL、AK、CN耐药率较高,分别为100.00%、96.10%、75.00%、58.18%、32.73%,对C、LEV、AMC、SAm、CAZ、CTX、FOX敏感率较高,分别为98.00%、95.06%、94.18%、94.18%%、93.80%、93.80%、92.73%,所有菌株对TZP、FEP、IPM、MEM、CIP、SXT、TE均100.00%敏感。结论食源性腹泻VP毒力基因携带率较高,主流菌株为O3:K6型,对抗生素的耐药率不高,临床治疗应注意抗生素的合理使用,防止耐药菌株的出现。

Objective To analyze the virulence genes,Serology and antimicrobial Susceptibility of Vibrio parahaemolyticus strains isolated from intestinal outpatients in Huzhou,Zhejiang Province,and provide a scientific basis for the prevention and control of local foodbomne diseases.Methods The virulence genes(tdh,trh,th)ang O and K antigen determination and antimicrobial Susceptibility of 170 Vibrio parhaemolyticus strains isolated from the feces of intestinal outpatients of the Fist People's Hospital of Huzhou,Zhejiang Province from 2015 to 2019 were tested.Results The 170 Vibrio parahaemolyticus strains all carried tdh and th genes,and no trh was detected.The serotype was detected most often in O3:K6.Among the 20 commonly used antibacterial drugs in clinical,the strains was resistant to AMP,KZ,PRL,AK,CN,which are 100.00%,96.10%,75.00%,58.18%,32.73%,and the sensitivity rates to C,LEV,AMC,SAM,CAZ,CTX,FOX were higher,which were 98%,95.06%,94.18%,94.18%,93.80%,93.80%,92.73%,all strains were sensitive to TZP,FEP,IPM,MEM,CIP,SXT,TE at 100.00%.Conclusion The virulence gene carrying rate of foodborme diarhea Vibrio parahaemolyticus in Huzhou area is high,the mainstream strain is O3:K6 type,and the resistance rate to antibiotics is not high.Reasonable use of antibiotics should be paid attention in clinical treatment to prevent resistant strains.Appear.