中国主栽樱花的分子系统发育分析

Molecular Phylogenetic Analysis of Cerasus Plants in China

以88份樱花为试验材料,利用核基因ITS和叶绿体间隔序列trnH-psbA进行测序,经过软件MEGA 6.0处理后,构建了系统发育树。结果表明,山樱群樱花品种系统发育比较混乱,这与其遗传背景比较复杂以及原始亲本系统发育关系较近有关,江户彼岸群和寒绯樱群的大多数品种能够聚类在一起,与山樱群品种能分得开,但涉及到很多杂交品种,ITS和trnH-psbA难以区分,表明DNA条形码技术难以应用到杂交品种的系统发育关系,此外,还可依据某些樱花品种特异的ITS或trnH-psbA序列长度对部分品种进行分子鉴定,也对部分种系不明的品种进行了修订。

To provide some molecular basis for the species classification and lineage location of cherry blossoms mainly planted in China,with 88 cherry blossoms,by nuclear gene ITS and chloroplast intergenic sequence,trnH-psbA were sequenced,and after processed by software MEGA 6,phylogenetic tree was constructed.The phylogenetic development was confused in Cerasus serrulata group,which is related to the complex genetic background and the close phylogenetic relationship among the primitive parental,meanwhile,it could be clustered together in most of the cultivars C.subhirtella group or C.campanulata group,and could be separated from the cultivars of the Cerasus serrulata group,however,it was difficult to distinguish in many hybrids using ITS and trnH-psbA,which indicated that DNA barcoding technology was difficult to apply to the phylogenetic relationship of hybrids,in addition,some cherry cultivars could be identified by ITS or trnH-psbA sequence length,and some unidentified varieties were also revised.