GC-MS法分析畲药十二时辰花中挥发油成分

GC-MS analysis of volatile oil from traditional She medicine Clematis Florida var. plena flower

目的对畲药十二时辰花Clematis florida. var. plena挥发油的主要化学成分进行分析研究,为十二时辰规模化合理栽培及质量标准的制定提供参考。方法采用水蒸气蒸馏法提取十二时辰花中的挥发油,采用GC-MS进行分析鉴定,并采用面积归一化法测定各成分的百分比含量。结果从十二时辰花挥发油中检出32种成分,鉴定出20种挥发油化学成分,其中含量高于1%的成分有13个,占比最多的5种成分分别为棕榈酸(26.94%)、叶绿醇(10.58%)、亚油酸(6.13%)、十五烷(4.54%)和正二十三烷(3.84%),以棕榈酸含量最高。结论采用GC-MS联用技术分析十二时辰花挥发油活性成分的主要化学组成,为推广十二时辰的种植、生产及进一步开发利用提供了科学依据。

Objective Clematis florida var. plena is a traditional She medicine. In this paper, we aim to study the main chemical components of essential oil of C. florida var. plena flower. The study will provide reference for large-scale reasonable cultivation and quality standard establishment of C. florida var. plena. Methods C. florida var. plena flower’s essential oil was extracted with steam distillation and it was identified by GC-MS. Area normalization method was used to measure the percentage of each components. Results In the experiment, 32 element were detected in essential oil of C. florida var. plena flower, among which 20 chemical components were identified. There are 13 chemical components over 1%, and the top five compounds are palmitic acid(26.94%), phytol(10.58%), linoleic acid(6.13%), pentadecane(4.54%) and n-tricosane(3.84%). Conclusion GC-MS was first used to analyze the main chemical components of C. florida var. plena’s flower essential oil. The study provides scientific basis for cultivation, production, development, utilization of C. florida var. plena.