苦郎树组培快繁技术研究

Tissue Culture and Rapid Propagation of Clerodendrum inerme

对苦郎树(Clerodendrum inerme)的组培快繁技术进行研究,以建立完整的离体快繁体系。以苦郎树的半木质化茎段为外植体,研究不同植物生长调节物质对腋芽诱导、增殖和生根的影响。结果表明,苦郎树茎段最佳灭菌方式为75%酒精处理30 s、0.1%HgCl2处理7 min,污染率18%。最佳诱导培养基为1/2 MS+1.0 mg/L 6-BA+0.15 mg/L NAA+0.5 mg/L IBA,诱导率90%;最佳继代培养基为1/2 MS+1.5 mg/L 6-BA+0.10 mg/L NAA,增殖倍数4.2;最佳生根培养基为1/2 MS+0.10 mg/L NAA,生根率93.33%。将生长健壮的植株移栽至珍珠岩、泥炭土和沙子(2∶2∶1)的混合基质中,成活率100%。

Tissue culture and rapid propagation of Clerodendrum inerme was studied in order to establish rapid propagation in vitro completely.Semi-lignified stem segments of C.inerme were used as explants and effects of different plant growth regulators on axillary buds induction,multiplication and rhizogenesis were studied.Results showed that the best sterilization method for stem segments was 75%alcohol treatment for 30 s,0.1%HgCl2 treatment for 7 min,and the pollution rate was 18%.The optimum induction medium was 1/2 MS+1.0 mg/L 6-BA+0.1 mg/L NAA+0.5 mg/L IBA,and induction rate was 90%.The optimum subculture medium was 1/2 MS+1.5 mg/L 6-BA+0.10 mg/L NAA,and multiplication ratio was 4.2.The optimum rooting medium was 1/2 MS+0.10 mg/L NAA and the rooting rate was 93.33%.Strong plants were transplanted to mixture substrate with perlite,peat soil and sand(2∶2∶1)and the survival rate was 100%.