棉花BFT基因的全基因组分析

Genome-wide analysis of BFT genes in cotton

BROTHER OF FT AND TFL1(BFT)被称为FLOWERING LOCUS T(FT)和TERMINAL FLOWER 1(TFL1)的兄弟,在植物开花通路中发挥着重要的调控作用。在盐胁迫下,拟南芥BFT蛋白在顶端分生组织中同bZIP转录因子FD互作,形成复合体调控开花转变。为了探索棉花BFT同源基因的功能,本研究基于棉花最近更新的基因组数据库,通过全基因组分析共鉴定了12个BFT基因,其中四倍体陆地棉和海岛棉中均发现4个BFT基因,二倍体亚洲棉和雷蒙德氏棉中各有2个BFT基因;核苷酸和氨基酸序列比对分析发现,棉花BFT基因结构和编码蛋白保守基序与其它植物的BFT高度一致,表明在进化过程中相当保守;组织表达结果显示GhBFT 1和GhBFT 2在陆地棉叶和花中表达量较高;胁迫诱导表达分析表明GhBFT 1和GhBFT2不同程度地响应盐、干旱、ABA、高温及低温胁迫,并具有相似的表达特征。酵母双杂交和双分子荧光互补实验证明,GhBFT1和GhBFT2均能与4个GhFD同源蛋白互作。该研究结果为深入探索GhBFT基因的功能奠定了一定基础。

BROTHER OF FT AND TFL 1(BFT)is known as the brother of FLOWERING LOCUS T(FT)and TERMINAL FLOWER 1(TFL1),and they play an important role in regulating flowering transition.Under salt stress,Arabidopsis BFT protein interacts with bZIP transcription factor FD in shoot apical meristem to form a complex to regulate flowering transition.To explore the function of cotton BFT homologous genes,12 BFT genes were identified by genome-wide analysis according to the recently updated cotton genome database,including four BFT in tetraploid G.hirsutum and G.barbadense,and two BFT in diploid G.arboretum and G.raimondii,respectively.Nucleotide and amino acid sequence alignment analysis showed that the structure and conserved motif of cotton BFT genes are highly consistent with those of other plants'BFT,indicating that cotton BFTs were conserved during the process of evolution.Tissue expression analysis revealed showed that GhBFT 1 and GhBFT 2 were highly expressed in leaves and flowers,and their transcription were induced in response to salt,drought,ABA,high temperature and low temperature stress with varied degrees,and similar expression patterns.Yeast two-hybrid and bimolecular fluorescence complementary assays showed that both GhBFT1 and GhBFT2 could interact with four GhFD proteins.The results of this study lay a foundation for further exploration of GhBFT genes function.