Klotho通过抑制氧化应激对肾小管上皮细胞低氧/再氧化损伤的影响被引量：2

Klotho’s influence on hypoxia/reoxidation damage of renal tubular epithelial cells by inhibiting oxidative stress

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摘要目的探讨Klotho蛋白对肾小管上皮细胞低氧/再氧化损伤的保护机制。方法体外培养小鼠近端肾小管上皮细胞(TCMK-1),根据是否给予低氧/再氧化刺激及Klotho蛋白干预分为对照组(无干预),低氧/再氧化损伤组(低氧24 h后再复氧不同时间),低氧/再氧化+Klotho干预组(给予Klotho蛋白预处理1 h后,低氧24 h后再复氧8 h)。Western blot法检测细胞Klotho蛋白的表达;Cyto Tox-ONETM法检测细胞乳酸脱氢酶(LDH)的释放水平,评估细胞损伤程度;DCFH-DA探针检测细胞内活性氧(ROS)水平。结果低氧/再氧化损伤组LDH释放率高于对照组,低氧/再氧化+Klotho治疗组LDH释放率低于低氧/再氧化损伤组(P <0.05)。同时,低氧/再氧化损伤组ROS水平高于对照组,低氧/再氧化+Klotho治疗组ROS水平低于低氧/再氧化损伤组(P <0.05)。结论 Klotho对肾小管上皮细胞低氧/再氧化损伤的保护作用可能与其抗氧化应激的作用机制相关。

作者邵驾宇应奇素钱盈盈杨秀费晓王鸣 SHAO Jiayu;YING Qisu;QIAN Yingying

机构地区杭州市老年病医院

出处《现代实用医学》 2020年第12期1458-1459,I0001,共3页 Modern Practical Medicine

关键词肾小管上皮细胞低氧/再氧化 KLOTHO 氧化应激

分类号 R692 [医药卫生—泌尿科学]

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