摘要
[Objectives]This study was conducted to compare and analyze the accuracy of computer-aided sperm analysis(CASA)and manual method for detecting the quality of fresh boar semen at room temperature.[Methods]Statistical methods such as analysis of variance,ZB score and Z score were used to compare the accuracy of five different brands of CASA systems and manual method to detect the vitality and density of fresh boar semen at room temperature.[Results]After setting the parameters of five CASA systems the same as follows:VCL(curvilinear velocity),VSL(straight-line velocity)≥5μm/s,STR(straightness)=VSL(straight-line velocity)/VAP(average path velocity)≥25%,the sperm motility of six parts of boar semen was tested at normal temperature using different brands of special fixed-volume slides with a uniform chamber height[(20±2)μm].There were no significant differences in sperm vitality detected by the five CASA systems(P>0.05).The ZB scores of the vitality obtained by observers or instrument engineers who did not have a job certificate from the quality inspection department showed that the results of three observers or instrument engineers were unsatisfactory(∣ZB∣>3),but there were no significant differences in vitality between the CASA systems and the inspector with a job certificate(P>0.05).Regarding sperm density detection,when the sperm density was less than 280×106/ml,there were no significant differences between the results displayed by the instruments and the results of the manual hemocytometer counting(P>0.05).[Conclusions]The accuracy of the CASA systems set to uniform parameters was consistent with the accuracy of the visual vitality obtained by an inspector with a job certificate.When the semen was diluted with 3%NaCl solution to a sperm density<280×10^6/ml,the sperm density detected by the CASA systems was consistent in reliability with that obtained by the hemocytometer detection.The CASA systems are faster and more efficient and objective than manual detection,and have the advantages of strong operability and easy promotion.
