摘要
To clarify the effect of the N deficit on the amount of square Bt insecticidal protein, different N application rates(0, 75, 150, 225, and 300 kg ha^–1) were imposed on the conventional cultivar Sikang 1(SK-1) and hybrid cultivar Sikang 3(SK-3) during 2015–2016 cotton growth seasons. Under different N application rates, the square number per plant, square volume and square dry weight reduced when the N rates decreased from conventional rate(300 kg ha^–1) to 0 kg ha^–1. And the square Bt protein content decreased accordingly. The analysis of N metabolism showed that soluble protein content, GPT and GOT activities decreased, free amino acid, peptidase and protease activities increased under N deficit. Correlation analysis indicated that the reduced Bt protein content under N deficit was related to altered N metabolism. In conclusion, square development and the amount of square Bt toxin both decreased under N deficit, indicating that promoting the square development under appropriate N application rate would also promote the insect resistance during squaring stage.
基金
supported by the National Natural Science Foundation of China (31901462 and 31671613)
the National Key Research and Development Program of China (2018YFD0100406 and 2018YFD1000907)
the Natural Science Foundation of the Jiangsu Higher Education Institutions,China (18KJB210013 and 17KJA210003)
the Natural Science Foundation of Jiangsu Province,China (BK20191439)。