维生素D受体基因多态性及儿童免疫力的相关性研究

Study on the relationship between vitamin D receptor gene polymorphism and children's immunity

目的探讨儿童免疫力与维生素D受体基因(Vitamin D receptor,VDR)的相关性,揭示VDR基因多态性和儿童免疫力的相关性,并研究其在免疫力低下发生发展中的作用机制。方法选取2018年1月~2020年1月在我院进行检查的0~14岁儿童80例,根据T淋巴细胞亚群检测,将儿童分为研究组(免疫力低下)和对照组(健康),每组各40例。收集各组儿童的年龄、身高等临床资料及外周血标本,运用化学发光法检测其血清中维生素D(VD)的浓度。设计引物用位点阻滞法,进行VDR多态性位点Apa Ⅰ、Bsm Ⅰ、Taq Ⅰ、Fok Ⅰ的检测。结果两组VDR多态性位点Bsm Ⅰ、Taq Ⅰ、Fok Ⅰ基因型比较,差异无统计学意义(P>0.05);两组Apa Ⅰ基因型的分布比较,差异有统计学意义(P<0.01)。研究组外周血清中25-OH VD水平低于对照组,差异有统计学意义(P<0.05)。研究组感冒次数多于对照组,差异有统计学意义(P<0.05),且感冒次数与VD水平呈负相关。结论 ApaⅠ基因多态性可能在免疫力低下的发病机制中发挥作用,Bsm Ⅰ、Taq Ⅰ、Fok Ⅰ基因多态性则与其无关。研究组VD水平低于对照组,提示血清VD水平可能对免疫力有增强作用。

Objective To explore the correlation between child immunity and vitamin D receptor gene(vitamin D receptor, VDR), to reveal the correlation between VDR gene polymorphism and child immunity, and to study its mechanism in development in the development of low immunity. Methods A total of 80 children aged 0-14 were selected from January 2018 to January 2020 in our hospital, according to the detection of T lymphocyte subsets, they were divided into research group(immunocompromised) and control group(healthy) with 40 children in each group. And the clinical data such as age and height, and the peripheral blood samples of children were collected. The concentration of vitamin D(VD) in serum was detected using chemiluminescence method. The VDR polymorphism sites Apa Ⅰ, Bsm Ⅰ, Taq Ⅰ, Fok Ⅰ were detected by site block method for primer design. Results There were no significant differences in the Bsm Ⅰ, Taq Ⅰ and Fok Ⅰ genotypes of VDR polymorphism sites between the study group and the control group(P>0.05). The distribution of Apa Ⅰ genotype in the two groups was statistically different(P<0.01). The 25-OH VD level in the peripheral serum of the study group was lower than that of the control group, and the difference was statistically significant(P<0.05). The number of colds in the study group was higher than that in the control group, and the difference was statistically significant(P<0.05). The number of colds and VD levels had a negative correlation trend. Conclusion The polymorphism of Apa Ⅰ gene may play a role in the pathogenesis of immunocompromise, but the polymorphism of Bsm Ⅰ,Taq Ⅰ, and Fok Ⅰ genes has nothing to do with it. The level of VD in the study group was lower than that in the control group, suggesting that the level of serum VD may enhance immunity.