茶油微胶囊的制备及其缓释性能

Preparation and slow release properties of camellia oil microcapsules

为提高茶油的存储稳定性,以乳清分离蛋白(WPI)与阿拉伯胶(GA)为复合壁材,转谷氨酰胺酶为固化剂,采用复凝聚法制备茶油微胶囊。首先,以凝聚率为指标,通过单因素实验确定最佳的壁材复凝聚参数;随后,以微胶囊形貌和包埋率为指标,通过单因素实验和响应面实验确定最佳的茶油微胶囊制备条件为:壁材质量分数2.0%(即WPI与GA壁材占全部混合物的质量分数)、壁材组分WPI与GA的质量比1∶1、芯壁质量比0.84∶1.00、复凝聚pH=4.3、复凝聚温度40℃和搅拌速度500 r/min,此时包埋率达89.67%。FTIR、SEM、粒径分布、休止角、过氧化值(POV)和TG结果表明,茶油微胶囊结构完整、粒径均一且具有良好的流动性、分散性、热稳定性和抗氧化性。体外缓释数据表明,茶油微胶囊在模拟胃液和肠液中的缓释机理均符合Ritger-Peppas缓释模型,符合人体消化吸收特性。茶油微胶囊化保护茶油的有效成分,扩展其使用范围,提高其消化吸收效果。

To increase the storage stability of camellia seed oil,camellia oil microcapsules were obtained by the complex coacervation method with whey protein isolate(WPI)and gum arabic(GA)as composite wall materials,and transglutaminase as curing agent.Firstly,the optimal complex coacervation parameters of wall material were determined by single factor experiment using agglomerate rate as evaluation index.Secondly,the optimal preparation conditions of camellia oil microcapsules were obtained by single factor experiments and response surface experiment using morphology and embedding rate of microcapsule as evaluation indexes.The optimum reaction conditions were as follows:mass fraction of wall materials 2.0%[based on the total mass of the mixture,m(WPI)∶m(GA)=1∶1],m(core material)∶m(wall material)=0.84∶1.00,p H of complex coacervation 4.3,temperature of complex coacervation 40℃,stirring speed 500 r/min.Under those conditions,the embedding rate reached 89.67%.FTIR,SEM,particle size distribution,angle of repose,POV value and TG results showed that the prepared camellia oil microcapsules had good structural integrity,particle size uniformity,fluidity,dispersibility,thermal stability and oxidation resistance.The sustained release data in vitro indicated that the slow-release mechanism of camellia oil microcapsules in both simulated gastric and intestinal juice were Ritger-Peppas releasing model,which is in accordance with human digestive and absorptive characteristic.The camellia oil microcapsules can protect the effective ingredient of camellia oil,expand its application range,and improve its digestion and absorption effect.