不同抗生素参与下呋喃唑酮代谢物在鲫鱼体内的残留消除规律

Residual Elimination of Furazolidone Metabolites in Crucian Carp under the Action of Different Antibiotics

本文研究了土霉素、恩诺沙星、磺胺间甲氧嘧啶分别与呋喃唑酮联合药浴给药后,呋喃唑酮代谢物3-氨基-2-恶唑烷酮(AOZ)在鲫鱼体内的残留消除规律。结果表明,呋喃唑酮在进入鲫鱼体内后迅速代谢成AOZ,停药0 h,呋喃唑酮对照组中鲫鱼肾脏、肝脏、肌肉中AOZ残留量便达到最高,分别为88.87、59.20、14.91μg/kg,平均消除速率为0.86、0.52、0.14μg/(kg·h);与对照组相比,土霉素-呋喃唑酮组鲫鱼肾脏、肝脏、肌肉中AOZ最大残留浓度明显增加,分别为165.40、125.29、28.69μg/kg,平均消除速率为1.71、1.17、0.31μg/(kg·h);而恩诺沙星-呋喃唑酮组鲫鱼肾脏、肝脏、肌肉中AOZ最大残留浓度与对照组相比显著减少,仅为46.70、41.64、9.69μg/kg,平均消除速率为0.36、0.31、0.03μg/(kg·h);磺胺间甲氧嘧啶-呋喃唑酮组中鲫鱼体内AOZ最大残留量及平均消除速率与对照组相比也有差异但变化不大。消除96 h后,各实验组鲫鱼体内仍有大量AOZ残留。本实验初步表明AOZ在鲫鱼体内难消除,且土霉素、恩诺沙星、磺胺间甲氧嘧啶能够不同程度的影响AOZ在鲫鱼体内的残留与消除,这为水产品质量安全监管和渔药残留研究提供了理论基础。

In this article,the residue elimination rule of furazolidone metabolite 3-amino-2-oxazolidinone(AOZ)in crucian carp was studied after oxytetracycline,enrofloxacin,sulfamethoxine and furazolidone were administered in combination with bath.The results showed that furazolidone was rapidly metabolized into AOZ after entering the crucian carp,after the drug was stopped for 0 hours,the furazolidone control group had the highest residual AOZ in the kidney,liver,and muscle of the crucian carp,which were 88.87,59.20,and 14.91μg/kg,respectively,with an average elimination rate.0.86,0.52,0.15μg/(kg·h),respectively.Compared with the control group,the maximum residual concentration of AOZ in the kidney,liver,and muscle of crucian carp in the oxytetracycline-furazolidone group was significantly increased,which were 152.46,125.39,8.69μg/kg,respectively,and the average elimination rate was 1.71,1.17,0.31μg/(kg·h),respectively.However,the maximum residual concentration of AOZ in the kidney,liver,and muscle of Carassius auratus in the enrofloxacin-furazolidone group was significantly reduced compared with the control group,only 33.68,49.77,9.69μg/kg,respectively,and the average elimination rate was 0.36,0.31,0.03μg/(kg·h),respectively.The maximum residual amount of AOZ and the average elimination rate of AOZ in the crucian carp in the sulfamonomethoxine-furazolidone group were also different from those in the control group,but there was no significant change.After 96 hours of elimination,a large amount of AOZ remained in the crucian carp in each experimental group.It was initially found that in this experiment,AOZ is difficult to be eliminated in crucian carp.Oxytetracycline,enrofloxacin,and sulfamonomethoxine can affect the elimination of AOZ in crucian carp to varying degrees,and provides a theoretical basis for aquatic product quality and safety supervision and fishery drug residue research.