乙型肝炎病毒血清学检验采用化学发光法与酶联免疫法的效果比较

Comparative Study on the Effect of Chemiluminescence and ELISA in Serological Test of Hepatitis B Virus

目的比较乙型肝炎病毒血清学检验采用化学发光法与酶联免疫法的效果。方法将2017年9月至2018年8月在我院就诊的孕产妇中确诊为乙型肝炎的100例患者纳为研究对象,根据随机抽签分组的方法将100例患者分为对照组与观察组,取两组患者的血液标本,对照组通过酶联免疫吸附法进行乙型肝炎病毒标志物的检测,观察组患者通过化学发光酶免疫分析法进行乙型肝炎病毒标志物的检测,比较两组的乙型肝炎e抗体、乙型肝炎e抗原、乙型肝炎表面抗原、乙型肝炎表面抗体、乙型肝炎核心抗体检测的阳性率。结果观察组的乙型肝炎e抗体检测的阳性率显著高于对照组(P<0.05);观察组的乙型肝炎e抗原检测的阳性率略高于对照组(P>0.05);观察组的乙型肝炎表面抗原检测的阳性率显著高于对照组(P<0.05);观察组的乙型肝炎表面抗体检测的阳性率略高于对照组(P>0.05);观察组的乙型肝炎核心抗体检测的阳性率高于对照组(P>0.05)。结论化学发光酶免疫分析法与酶联免疫吸附法虽然在对乙型肝炎e抗原、乙型肝炎表面抗体、乙型肝炎核心抗体的检测阳性结果上无显著差异,但化学发光酶免疫分析法对乙型肝炎e抗体和乙型肝炎表面抗原的检测效果更优,阳性率更佳。

Objective To compare and analyze the effect of chemiluminescence and ELISA in serological test of hepatitis B virus.Methods From September 2017 to August 2018,100 pregnant and lying-in women diagnosed with hepatitis B in our hospital were divided into control group and the observation group according to the method of random lot grouping.Blood samples were taken from the two groups.The control group was tested for hepatitis B virus markers by enzyme-linked immunosorbent assay.Chemiluminescent enzyme immunoassay was used to detect hepatitis B virus markers in the observation group.The positive rates of hepatitis B e antibody,hepatitis B e antigen,hepatitis B surface antigen,hepatitis B surface antibody and hepatitis B core antibody in the two groups were taken as observation indexes.Results The positive rate of hepatitis B e antibody detection in the observation group was signifi cantly higher than that of the control group(P<0.05);the positive rate of hepatitis B e antigen detection in the observation group was slightly higher than that of the control group(P>0.05).The positive rate of hepatitis B surface antigen detection in the observation group was signifi cantly higher than that of the control group(P<0.05);the positive rate of hepatitis B surface antibody detection in the observation group was slightly higher than that of the control group(P>0.05);the positive rate of hepatitis B core antibody detection in the observation group was higher than that of the control group(P>0.05).Conclusion Chemiluminescence enzyme immunoassay and enzyme-linked immunosorbent assay have no signifi cant diff erence in the positive results of hepatitis B e antigen,hepatitis B surface antibody and hepatitis B core antibody,but chemiluminescence enzyme immunoassay has a better eff ect in the detection of hepatitis B e antibody and hepatitis B surface antigen,and the positive rate is better.