不同微生物预处理对煤转化生物气的促进效果对比

Comparison on promotion effect of different microbial pretreatments on conversion of coal to biogas

为了查明不同种类微生物预处理对煤转化生物气的促进效果,选用绿孢链霉菌、白腐菌、假单胞菌3类菌种分别对褐煤进行预处理和厌氧发酵试验。通过生物产气效果检测、Gompertz方程模拟和扫描电子显微镜(SEM)方法探讨不同种类微生物预处理对产气的影响及内在原因。结果表明:在CH4的总产气量方面,与褐煤原煤相比绿袍链霉菌、白腐菌和假单胞菌预处理后的褐煤都有大幅的增加,增幅各达到了33.86%、165.28%、69.18%;拟合可知绿孢链霉菌、白腐菌、假单胞菌相比褐煤的最大比产甲烷量都有增加,分别为14.61%、154.49%和96.10%,微生物预处理对煤转化生物气的促进效果依次为白腐菌、绿孢链霉菌和假单胞菌。不同微生物的体积及其附着特征对预处理效果有重要影响,体积较小的菌种预处理效果较好,且预处理促使煤的孔-裂隙和表面粗糙度都显著增加,为后期厌氧发酵菌作用煤提供了便利条件。

In order to find out the promotion effect of different kinds of microbial pretreatment on the conversion of coal biogas,lignite was pretreated with three different types of strains including streptomyces viridosporus,phanerochaete chrysosporium and pseudomonas,followed by measurement of gas production and composition after fermentation with coal seam methanogenic flora.The effects of different kinds of microbial pretreatment on total gas production and composition,as well as the coal surface structure were investigated by biogas production effect detection,Gompertz equation simulation and scanning electron microscopy(SEM).The results show that compared to raw original lignite,the total methane production of lignite pretreated by streptomyces viridosporus,phanerochaete chrysosporium and pseudomonas increased by 36.86%,165.28%and 69.18%,respectively.The fitting data demonstrated that maximum specific methane production of streptomyces viridosporus,phanerochaete chrysosporium and pseudomonas have increased 14.61%,154.49%and 96.10%,respectively than that of raw original lignite.The effect of microbial pretreatment on coal-to-biogas conversion from strong to weak is followed by phanerochaete chrysosporitum,pseudononas and streptomyces viridosporus.The volume and adhesion characteristics of the different microorganisms have an important influence on the pretreatment effect.The smaller the volume of the strain,the better the pretreatment effect,and the pretreatment promotes the pore-fissure and surface roughness of the coal to increase significantly,and providing convenient conditions for the later anaerobic fermentation bacteria to act on coal.