摘要
目的了解云南省伤寒、副伤寒沙门菌脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)分子分型及耐药状况。方法参照中国细菌性传染病分子分型实验室监测网络PulseNetChina的沙门菌PFGE分子分型方法进行分子分型。分析耐药板最低抑菌浓度(minimal inhibitory concentration,MIC)值,根据美国临床实验室标准化协会(Clinical and Laboratory Standard Institute,CLSI)的相应标准得出敏感、中介、耐药(S、I、R)结果。结果36株伤寒、副伤寒沙门菌呈22种PFGE带型,有5组优势带型,组之间菌株有明显的时间、地域同源性。对萘啶酸耐药率最高,为55.56%,对庆大霉素最敏感,敏感率100%。此外有16.68%菌株对3类以上抗菌素耐药。结论云南省伤寒、副伤寒沙门菌分子分型结果提示5~8月存在甲型副伤寒局部爆发,应加强甲型副伤寒疾病的监测。云南省伤寒及副伤寒对萘啶酸耐药率最高,对庆大霉素最敏感,菌株存在多重耐药性。
Objective To understand the molecular typing and drug resistance of Salmonella typhi and paratyphoid pulsed field gel electrophoresis(PFGE)in Yunnan province.Methods Molecular typing was carried out by referring to the PFGE molecular typing method of Salmonella by PulseNet China molecular typing laboratory monitoring network for bacterial infectious diseases.The minimal inhibitory concentration(MIC)was analyzed,and the results of sensitivity(S),mediator(I)and drug resistance(R)were obtained according to the relevant standards of Clinical and Laboratory Standard Institute(CLSI).Results Totally 36 strains of Salmonella typhi and paratyphi showed 22 PFGE bands and 5 groups of dominant bands,with obvious temporal and regional homology among strains.The highest resistance rate was 55.56%to nalidixic acid and the most sensitive to gentamicin with 100%sensitivity.In addition,16.68%strains were resistant to more than 3 classes of antibiotics.Conclusion The results of molecular typing of Salmonella typhi and paratyphoid in Yunnan province indicate that paratyphoid A occurres locally from May to August,so the surveillance of paratyphoid A should be strengthened.Yunnan Salmonella typhi and Salmonella paratyphoid have the highest drug resistance rate to Nalidixic Acid(NAL)and the most sensitive to gentamicin(GEN),and the strain has multiple drug resistance.
作者
田云屏
邹颜秋硕
任翔
田云峰
TIAN Yun-Ping;ZOU Yan-Qiu-Shuo;REN Xiang;TIAN Yun-Feng(Yunnan Center for Disease Control and Prevention,Kunming 650022,China;Yunnan Press and Publication Industry Technology Center,Kunming 650030,China)
出处
《食品安全质量检测学报》
CAS
2020年第24期9434-9439,共6页
Journal of Food Safety and Quality
关键词
伤寒沙门菌
副伤寒沙门菌
脉冲场凝胶电泳
耐药
Salmonella typhi
Salmonella paratyphi
pulsed field gel electrophoresis
drug resistance