葱白提取物对心肌缺血再灌注损伤模型大鼠心肌细胞凋亡的作用及机制研究

Effect and Mechanism of Fistular Onion Bulb Extraction on Cardiomyocyte Apoptosis in Ischemia-Reperfusion Model Rats

目的探讨葱白提取物(FOB)对心肌缺血再灌注损伤模型(I/R)大鼠心肌细胞凋亡的保护作用及机制。方法36只雄性SD大鼠随机分为sham组(n=12)、I/R组(n=12)和FOB组(n=12)。结扎左冠状动脉前降支45 min再灌注。多导生理记录仪记录左心室功能变化,TUNEL染色观察心肌细胞凋亡情况,计算心肌细胞凋亡率;Western blot检测心肌梗死区,梗死边缘区,未梗死区pp38、p53蛋白表达;RT-PCR检测心肌组织p38、p53 mRNA表达量。结果与sham组相比,I/R组心功能显著受损,同时细胞凋亡率增加,p-p38、p53蛋白表达量显著升高,p53 mRNA相对表达水平显著升高(P<0.05);FOB预处理后改善了大鼠心功能,同时细胞凋亡率降低,p-p38、p53蛋白表达量下降,p53 mRNA相对表达水平下调(P<0.05)。结论FOB具有改善I/R模型大鼠心功能作用,其机制与调节p38 MAPK信号通路蛋白表达、抗心肌细胞凋亡相关。

Objective To explore the protective effect and mechanism of fistular onion bulb(FOB)extraction on myocardial cell apoptosis in ischemia-reperfusion(I/R)model rats.Methods Thirty-six male SD rats were randomly divided into the sham group(n=12),the I/R group(n=12)and the FOB group(n=12).The anterior descending branch of the left coronary artery was ligated and reperfused for 45 minutes.Multi-channel physiological recorder recorded the changes of left ventricular function,TUNEL staining was used to observe the myocardial cell apoptosis and calculate the myocardial cell apoptosis rate;Western blot was used to detect the expression of p-p38,p53 protein in myocardial AAR,BZ,andRA;RT-PCR detected myocardial tissue p38,p53 mRNA expression. Results Comparedwith the sham group,the heart function of the I/R group was significantly impaired,theapoptosis rate increased,the expression levels of p-p38 ,and p53 protein were significantlyincreased,and the relative expression level of p53 mRNA was significantly increased(P <0. 05);FOB pretreatment improved the rat heart function,the apoptosis rate decreased,theexpression levels of p-p38,and p53 protein decreased,and the relative expression level ofp53 mRNA was downregulated (P < 0. 05). Conclusion The FOB can improve thecardiac function of I/R rats,which is related to the regulation of p38 MAPK signal pathwayprotein expression and anti-cardiomyocyte apoptosis.