大黄素治疗对去卵巢大鼠骨量流失的保护作用机制研究

Protective mechanism of emodin on bone loss in ovariectomized rats

目的探讨大黄素(emodin,DHS)对去卵巢大鼠骨量流失的影响,并探索可能的机制。方法通过双侧去卵巢建立骨质疏松大鼠模型;随后随机分为假手术组(Sham)、去卵巢组(OVX)以及大黄素组(DHS),每组10只;其中DHS组大鼠接受大黄素[90 mg/(kg·d)]治疗12周;待治疗结束后使用Micro-CT、HE染色切片、骨代谢指标、以及蛋白质印迹观察治疗效果以及可能的机制。结果治疗12周后,与OVX组相比,Micro-CT和HE染色切片结果显示DHS组的大鼠骨小梁数量和骨密度得到明显改善。DHS组大鼠BMD、TV/BV、Tb.N、Tb.Th和Tb.Sp较OVX组明显改善(P<0.05)。与OVX组相比,DHS组的BALP水平明显升高(P<0.05);而TRACP-5b和β-CTX水平显著降低(P<0.05)。和OVX组比较,DHS组OPG表达水平上调(P<0.05),而RANKL和β2AR表达水平下调(P<0.05)。结论大黄素可以通过降低β2AR表达和激活OPG/RANKL信号通路介导对去卵巢大鼠骨量流失的保护作用。

Objective To explore the effect of emodin(DHS)on bone loss in ovariectomized rats and to explore its possible mechanism.Methods In this study,osteoporosis rat model was established with bilateral ovariectomy.Rats were then randomly divided into a sham operation group(Sham),ovary removal group(OVX),and emodin group(DHS),with 10 rats in each group.Rats in DHS group received 90 mg/(kg·d)of emodin for 12 weeks.After the treatment,micro-CT,HE stained sections,bone metabolism indicators,and Western blotting were used to observe the treatment effect and possible mechanism.Results After 12 weeks of treatment,compared with the OVX group,the result of Micro-CT and HE staining in DHS group showed that the number of trabeculae and bone mineral density were significantly improved.BMD,TV/BV,Tb.N,Tb.Th,and Tb.Sp in DHS group were significantly improved compared with those in OVX group(P<0.05).Compared with the OVX group,BALP level in DHS group increased significantly(P<0.05).The levels of TRACP-5b andβ-CTX reduced significantly(P<0.05).Compared with the OVX group,the expression level of OPG in DHS group increased(P<0.05),while the expression levels of RANKL andβ2AR decreased(P<0.05).Conclusion Emodin mediates the protection of bone loss in ovariectomized rats by reducingβ2AR expression and activating OPG/RANKL signaling pathway.