牛磺酸治疗通过激活自噬介导对老年大鼠骨量和骨强度的保护作用

Protective effects of taurine treatment on bone mass and bone strength in aged rats through activation of autophagy

目的探讨牛磺酸(NHS)对老年大鼠骨量流失的影响,并探讨可能的机制。方法将30只大鼠随机分为对照组(CON)、模型组(MOD)以及牛磺酸组(NHS),每组10只;其中NHS组大鼠每天接受牛磺酸(2 g/kg)治疗12周;待治疗结束后通过Micro-CT检测、HE染色切片、血清指标、蛋白质印迹观察治疗效果,探讨可能的机制。结果治疗12周后,与MOD组相比,三点弯曲试验、Micro-CT和HE染色切片结果显示NHS组大鼠的骨小梁数量、骨强度和骨密度(bone mineral density,BMD)得到明显改善。NHS组大鼠最大载荷和弹性模量、BMD、TV/BV、Tb.N、Tb.Th和Tb.Sp较OVX组明显改善(P<0.05)。和MOD组比较,NHS治疗后大鼠血清BLAP、P1NP、TRACP-5b和β-CTX水平明显降低,组间差异有统计学意义(P<0.05)。和MOD组比较,NHS组Runx2、BMP2、Beclin-1和LC3Ⅱ/LC3-Ⅰ表达水平明显上调,而P62表达水平显著下调,比较差异有统计学意义(P<0.05)。结论NHS可能通过激活自噬,从而对年龄引起的骨量丢失起到保护作用。

Objective To explore the effect of taurine(NHS)on bone loss in aged rats and explore the possible mechanisms.Methods Thirty rats were randomly divided into control group(CON),model group(MOD)and taurine group(NHS),10 rats in each group.Of which,the rats in the NHS group received taurine(2 g/kg)daily 12 weeks.After treatment,observe the treatment effect and possible mechanism by Micro-CT detection,HE stained sections,serum indicators,and Western blot.ResultsAfter 12 weeks of treatment,compared with the MOD group,the result of the three-point bending test,Micro-CT and HE staining showed that the number of trabeculae,bone strength and bone density in the NHS group were significantly improved.The maximum load and elastic modulus,BMD,TV/BV,Tb.N,Tb.Th and Tb.Sp of the NHS group were significantly improved compared with the OVX group(P<0.05).Compared with the MOD group,the serum levels of BLAP,P1NP,TRACP-5b,andβ-CTX in rats after NHS treatment were significantly reduced,and the differences between the groups were statistically significant(P<0.05).Compared with the MOD group,the expression levels of Runx2,BMP2,Beclin-1,and LC3Ⅱ/C3-Ⅰin the NHS group were significantly increased,while the expression levels of P62 were significantly decreased,and there was a statistical difference(P<0.05).Conclusion NHS may protect age-affected bone destruction by activating autophagy.