玉米须多糖的乙醇沉淀分离及体内免疫调节作用研究

Isolation of polysaccharide from Stigma maydis by ethanol precipitation and enhanced efficacy of immunization in vivo

运用乙醇沉淀分离方法,将玉米须超滤多糖(corn silk polysaccharide,CSP)分级纯化为3种多糖CSP-1、CSP-2和CSP-3,并研究纯化后多糖对小鼠体液免疫功能、细胞免疫功能和主要器官的影响。结果显示,CSP主要由不溶于体积分数80%乙醇的高分子量多糖CSP-2组成,其含量占CSP的近一半。活性研究发现,玉米须多糖CSP以及2种分级多糖CSP-1和CSP-2可以有效提高小鼠抗体产生量,提高体液免疫能力;也可以有效激活巨噬细胞,提高其吞噬能力,增强细胞免疫能力;而CSP-3仅能提高体液免疫能力。CSP、CSP-1和CSP-3对小鼠胸腺和脾的生长有促进作用。4种多糖对小鼠心脏、肾、肺、肝和脑的生长没有显著影响。组间活性比较显示,CSP-1调节体液免疫功能优于CSP-2和CSP-3,而CSP-2对细胞免疫功能的调剂作用明显优于CSP和阳性对照。

By ethanol precipitation,the polysaccharide(CSP)extracted by ultrafiltration from Stigma maydis was isolated to three fractions,CSP-1,CSP-2 and CSP-3.The effect of this four kinds of polysaccharides on humoral and cellular immune functions and mainly organs in mice were studied.The results showed that CSP mainly contained CSP-2 which was precipitated by 80%ethanol.CSP,CSP-1 and CSP-2 could effectively improve antibody production and humoral immunity,activate macrophage,improve macrophage phagocytosis,but CSP-3 only could improve humoral immunity.CSP,CSP-1 and CSP-3 could increase immune organs index in mice.But all kinds of polysaccharides could not increase the heart,kidney,lung,liver and brain growing.Compared with each other,CSP-1 has a better activating effect on humoral immunity function than CSP-2 and CSP-3.CSP-2 has a better activating effect on cellular immune function than CSP and the positive control.