采用核酸酶酶解联合离子交换树脂吸附开发低嘌呤腐竹

Dried bean curd sticks with low purine prepared by nuclease enzymolysis combined with ion exchange resin adsorption

为研究开发一种低嘌呤腐竹,将树脂吸附和核酸酶酶解核酸2种措施相结合,对豆浆中的嘌呤类物质进行吸附,通过正交试验,确定吸附效果最强的树脂结合核酸酶酶解吸附的最佳技术条件。实验结果表明,树脂NUF20是最佳嘌呤吸附树脂,对嘌呤核苷酸吸附率达98. 40%,树脂NUF20结合核酸酶酶解吸附豆浆中嘌呤的最佳技术条件为核酸酶用量26. 67 U/m L,树脂添加量0. 2 g/m L,反应温度70℃下搅拌吸附1 h。与对照组相比,经过树脂NUF20结合核酸酶酶解技术处理后的豆浆加工的腐竹产品,第1~5张腐竹的嘌呤含量分别下降89. 70%、88. 28%、76. 69%、72. 03%和65. 62%。研究将树脂吸附和核酸酶酶解2种措施结合,采用优化的工艺条件,有效降低了腐竹中的嘌呤含量。实验结果为开发针对高血尿酸症等特殊人群的低嘌呤腐竹提供了技术基础,同时也为其他降嘌呤豆制品的研究及加工提供了理论参考。

In order to develop dried bean curd sticks with low purine,resin adsorption combined with nuclease enzymatic hydrolysis of nucleic acid were used to remove purine substances in soybean milk.The optimal conditions for the adsorption of resin combined with nuclease hydrolysis were determined by orthogonal experiment.The results showed that NUF20 resin was the best adsorption resin with the adsorption rate of 98.40%on purine and nucleotide.The optimum adsorption condition was obtained when soybean milk was stirred at 70℃for 1 h after mixed with 0.2 g/mL resin and 26.67 U/mL nuclease.After treatment,the purine content of the dried bean curd sticks in the 1 st to 5 th sheets decreased by 89.70%,88.28%,76.69%,72.03%and 65.62%,respectively.The research combined resin adsorption and nuclease digestion under optimal technical conditions to effectively reduced the purine content of dried bean curd sticks.It provides a technical basis for the development of dried bean curd sticks with low purine content for the special population such as hyperuricemia persons,also it provides a theoretical reference for the research and processing of other low purine bean products.