生物-化学法合成维生素D的研究进展

Advances on synthesis of vitamin D by bio-chemical method

维生素D是一种脂溶性类固醇激素,在平衡新陈代谢、维持机体健康等方面具有不可忽视的作用。传统的工业生产方法已无法满足日益增长的市场需求,而基于合成生物学方法改造细胞合成前体甾醇,再通过半化学法合成维生素D则是解决当前供需矛盾的有效手段之一。文章对生物-化学法合成维生素D的研究进展进行了概述,着重介绍了动态调控、亚细胞工程、定向进化等代谢工程技术在生物合成关键前体甾醇上的应用,并对今后工业化合成维生素D的研究方向进行了思考与展望。

Vitamin D is a fat-soluble steroid hormone precursor,which plays an important role in balancing metabolism and maintaining body health.At present,the yield of traditional industrial production methods cannot satisfy the market demand.While,applying synthetic biology to synthesize sterol and using semi-chemical method to synthesize vitamin D is one of the effective ways to solve the contradiction.The research progress of the synthesis of vitamin D by bio-chemical method were reviewed,and dynamic regulation,metabolic compartmentalization and subcellular engineering were especially focused on.Besides,this review puts forward the possible challenges and solutions in the future production.