低浓度臭氧降解小麦呕吐毒素技术初探

STUDY ON A DEGRADATION TECHNOLOGY OF DEOXYNIVALENOL IN WHEAT WITH LOW CONCENTRATION OZONE

基于呕吐毒素(DON)在小麦籽粒中的分布特性,从粮食生产实际出发,探寻一种低浓度臭氧降解粮食呕吐毒素的技术。实验结果表明,小麦中的呕吐毒素主要分布在籽粒皮层部位,有利于与臭氧结合反应。3 mg/L臭氧对主要分布在小麦原粮表皮外侧的呕吐毒素有较明显的去毒效果,呕吐毒素降解率可达50%,同时对小麦面筋含量基本无影响,并能有效降低霉菌总数(至少1个数量级)。但实仓储存的原粮水分含量低,会极大限制臭氧在籽粒中的进一步渗透,去毒效果受限于呕吐毒素在籽粒中的分布情况。此外,液质联用(UPLC-MS)和核磁共振(NMR)分析结果表明,臭氧对呕吐毒素的主要降解产物为己二酸二甲酯,是一种低毒性物质。本研究结论对提高粮食安全储藏水平和探索新型绿色储粮技术具有重要的现实意义。

A degradation technology of Deoxynivalenol(DON) in wheat with low concentration ozone(O3) was explored practically based on the distribution of DON in wheat. The results showed that DON was mainly concentrated on the episperm of wheat, which was conducive to the combination of DON and O3. Meanwhile, There was an evident degradation effect on DON of which degradation rate could reach about 50% after 3 mg/L O3 treatment for 8 h in the lab and 96 h in the barn when DON was mainly distributed on the outside of wheat episperm. Meanwhile, 3 mg/LO3 treatment did not affect the original quality indexes of grains basically, but could effectively reduce the total fungal count in grains by 1 cycle logcfu/g at least. However, the moisture content of wheat stored in the grain depot was usually low, which would limit the infiltrating of O3 into wheat grains. In addition, the results based on UPLC-MS and NMR analysis indicated that the final degradation product of DON treated by 3 mg/L O3 was dimethyl adipate, which was a low toxic substance. The research conclusions had an important practical significance for improving the safety level of grain storage, as well as exploring a new "green grain storage" technology.