基于网络药理学的威灵仙-急性子药对治疗食管癌的机制研究

Mechanism of"Radix clematidis-impatientis semen"in the treatment of esophageal cancer based on network pharmacology

目的:基于网络药理学,探索威灵仙-急性子配伍治疗食管癌的作用机制。方法:通过TCMSP数据库查找筛选威灵仙、急性子的活性成分,并结合文献进行补充,利用Swiss Target Prediction平台预测活性成分作用靶点;通过Genecards、TTD、DisGeNet数据库收集食管癌的主要靶点,利用韦恩图取交集可获得威灵仙-急性子药对治疗食管癌的关键靶点。使用Cytoscape3.7.2建立威灵仙-急性子药对治疗食管癌的“药物-活性成分-疾病-靶点”网络,联合String平台构建关键靶点PPI网络,找出核心靶点。在Metascape平台对关键靶点进行GO和KEGG富集分析,并绘制“活性成分-靶点-通路”网络图。结果:威灵仙-急性子药对中有槲皮素、山柰酚、3-表齐墩果酸、齐墩果酸等17个活性成分,作用于379个药物靶点,与2396个食管癌靶点相映射得到作用靶点178个,包括PIK3CA、PIK3R1、SRC、MAPK1、MAPK3等,KEGG富集分析主要涉及PI3K-Akt、Rap1、Ras、VEGF等信号通路。结论:本研究初步探讨了威灵仙-急性子药对治疗食管癌的潜在作用机制,为后续实验研究提供了依据与思路。

Objective:To explore the mechanism of"Radix Clematidis-Impatientis Semen"in the treatment of esophageal cancer based on network pharmacology.Methods:The active components of Radix Clematidis and Impatientis Semen were searched and selected through the TCMSP database,and supplemented by the literature.The targets of active components were predicted by Swiss Target Prediction platform.The main targets of esophageal cancer were obtained by Genecards,TTD and DisGeNet databases,and the key targets of“Radix Clematidis-Impatientis Semen”for the treatment of esophageal cancer were obtained by using Venn diagram analysis.The"drug-active ingredient-disease-target"network of“Radix Clematidis-Impatientis Semen”in the treatment of esophageal cancer was constructed with the help of Cytoscape3.7.2,and the PPI network of key target was built by using STRING platform and Cytoscape software to find the core target.Metascape platform was used for GO and KEGG enrichment analyses of key targets,and the network diagram of"active component-target-pathway"was drawn.Results:There were 17 active components such as quercetin,kaempferol,3-epioleanolic acid and oleanolic acid in“Radix Clematidis-Impatientis Semen”,with 379 drug targets.A total of 178 targets were obtained by mapping with 2396 disease targets of esophageal cancer,including PIK3CA,PIK3R1,SRC,MAPK1,and MAPK3.KEGG enrichment analysis mainly involved PI3K-Akt,Rap1,Ras,and VEGF signaling pathways.Conclusion:This study preliminarily explores the potential mechanism of Radix Clematidis-Impatientis Semen in the treatment of esophageal cancer and provides a basis and new insight into further experimental research.