TRIM21在胃癌诊断和预后中的价值

Diagnostic and prognostic value of TRIM21 in gastric cancer

目的探讨三结构域蛋白家族成员21(TRIM21)在胃癌中的表达及其与胃癌临床病理特征和临床预后的关系。方法应用公共数据库分析TRIM21在胃癌组织中的表达水平,分析其表达与胃癌临床预后的关系;通过基因富集分析(GSEA)TRIM21可能参与的信号通路;采用免疫组织化学染色方法检测TRIM21在80例胃癌组织及30例癌旁组织中的表达,并分析其表达与胃癌临床病理特征的关系。结果TRIM21在胃癌组织中呈显著低表达且TRIM21低表达的胃癌患者临床预后明显较差(均P<0.05);GSEA分析证明TRIM21参与胃癌患者辅助性T细胞分化的调控[P<0.0001,矫正后的P值(FDR)<0.0001]。结论TRIM21在胃癌组织中呈低表达且标志着更差的临床预后;TRIM21参与辅助性T细胞分化的调控并对胃癌的发生发展具有负性调控作用。

Objective To investigate the expression of tripartite motif 21(TRIM21)in gastric cancer tissues and its relationship with clinical pathological characteristics and clinical prognosis.Methods Public database was used to analyze the expression level of TRIM21 in gastric cancer tissues and the relationship between its expression and clinical prognosis.Gene set enrichment analysis(GSEA)was used to analyze the signaling pathways that TRIM21 might participate in.The expressions of TRIM21 in 80 gastric cancer tissues and 30 para-cancer tissues were detected by immunohistochemical staining,and the relationship between TRIM21 expression and clinicopathologic characteristics was analyzed.Results TRIM21 was significantly low-expression in gastric cancer tissues,and the clinical prognosis of patients with low TRIM21 expression was significantly worse(P<0.05);GSEA showed that TRIM21 was involved in the regulation of helper T cell differentiation in gastric cancer patients(P<0.0001,FDR<0.0001).Conclusions TRIM21 is poorly expressed in gastric cancer tissues and indicates the poor clinical prognosis.Moreover,TRIM21 is involved in the regulation of helper T cell differentiation and has a negative regulatory effect on the occurrence and development of gastric cancer.