摘要
选用白色耐高温灰树花(Grifola frondosa) Gr0001+3与性状优良的5个栽培菌株进行配对,得到13株杂交子作为试验菌株,研究温度和pH对不同杂交子生长的影响,并对杂交子进行分子鉴定。试验筛选出5株生长较快的杂交子,杂交子的菌丝最适生长温度为25℃,最适生长pH为5。共发现6种可以用于杂交子鉴定的RAPD和ISSR引物,并成功鉴定了其中7个杂交子菌株。为灰树花工厂化栽培专用菌株的生长条件控制、杂交子鉴定提供理论依据和实用方法。
Thirteen Grifola frondosa hybrids were obtained by pairing white high temperature resistant G. frondosa Gr0001+3 with five cultivated strains with excellent traits. These hybrids were used to study the effects of temperature and pH on the growth of different hybrids, and molecular identification. Initially five G. frondosa hybrids with maximum growth rate were screened. These hybrids showed optimum growth at 25°C, and pH 5. On the basis of six suitable RAPD and ISSR primers, seven hybrids were successfully differentiated and identified. The experiment results provided theoretical basis and practical method for optimum growth conditions and hybrid strains identification in G. frondosa.
作者
权新华
武鑫
温蝶
温志强
QUAN Xin-hua;WU Xin;WEN Die;WEN Zhi-qiang(Mycological Research Center of Fujian Agricultural and Forestry University,Fuzhou 350002,China;Jiaxing Academy of Agricultural Science,Jiaxing 314016,China)
出处
《中国食用菌》
北大核心
2020年第10期42-45,共4页
Edible Fungi of China
基金
2016年福建省科技厅引导项目(KJC16011A)
2015年福建农林大学校创新项目(KF2015047)。
关键词
灰树花
杂交子
分子鉴定
Grifola frondosa
hybrids
molecular identification
