同期神经化增强髂骨瓣术后骨髓间充质干细胞的活性

Simultaneous innervation revitalizes bone marrow mesenchymal stem cells from postoperative iliac grafts

目的:比较神经化髂骨瓣和传统髂骨瓣重建下颌骨缺损术后,移植骨块骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMMSCs)的干细胞活性。方法:游离血管化骼骨瓣移植修复下颌骨缺损术后半年,从移植骨骨髓体外分离、培养BMMSCs,通过集落形成观察、Brdu摄入实验、群体倍增时间、体外成骨茜素红染色法、裸鼠皮下成骨法检测BMMSCs增殖、自我更新及成骨分化能力。采用SPSS 24.0软件包对数据进行统计学分析。结果:神经化骼骨移植术后分离、培养的BMMSCs的集落形成、增殖、群体倍增时间、体外及体内成骨分化能力均显著高于非神经化组(P<0.05)。结论:神经化髂骨瓣重建下颌骨缺损可以增强BMMSCs的自我更新、增殖、成骨分化等潜能,有助于维持移植骨的术后内环境稳定,减少术后移植骨吸收。

PURPOSE:To compare the osteogenic potential of bone marrow mesenchymal stem cells(BMMSCs)from innervated iliac graft bone flap and traditional one for reconstructing mandibular defects.METHODS:Graft bone marrow samples were harvested 1 year after free vascularized iliac reconstruction of mandibular defects,with or without innervation through simultaneous nerve anastomosis.BMMSCs were isolated and cultured in vitro.Colony forming units-fibrosis observation,Brdu incorporation assay,population doubling,Alizarin red staining for in vitro calcified nodule formation and in vivo assay of subcutaneous osteogenesis in nude mice were used to detect BMMSCs proliferation,self-renewal and osteogenic differentiation capabilities,respectively.SPSS 24.0 software package was used for statistical analysis.RESULTS:Colony formation,proliferation,population doubling and osteogenic differentiation abilities of BMMSCs from innervated group were significantly higher than those from non-innervated group(P<0.05).CONCLUSIONS:Simultaneous innervation of free vascularized iliac during reconstruction of mandibular defects may maintain self-renewal and osteogenic differentiation potentials of BMMSCs in graft bones,thereby maintaining bone homeostasis and reducing postoperative graft bone resorption.