酿酒酵母酚类抑制物耐受性脂质组学研究

Lipidomics Analysis of Saccharomyces cerevisiae with Tolerance to Phenolic Inhibitors

通过脂质组学分析方法从细胞膜磷脂分布方面探究适应进化酿酒酵母酚酸耐受性机制。主要利用高效液相色谱-质谱(LC-MS)对酚酸胁迫下适应进化菌株和原始菌株脂质成分检测并进行统计学比较分析。检测出565种脂质代谢物,包含细胞膜磷脂185种。相比初始菌株,适应进化菌株细胞膜中磷脂酰胆碱(PC)、磷脂酰乙醇胺(PE)和磷脂酰肌醇(PI)类磷脂分子相对含量增加,含有长链(C32-C36)和双不饱和脂酰链的磷脂分子含量增加。统计学分析表明显著性差异磷脂分子主要为含有长链不饱和脂酰链的PC和PE类磷脂分子。推测适应进化菌株通过膜磷脂重塑提高细胞膜完整性,对酚类抑制物起到选择性屏障作用,从而保持细胞活性。

The mechanism of the adapted Saccharomyces cerevisiae tolerating to phenolic inhibitors was analyzed by lipidomics from the phospholipid profiles on cell membrane.The lipids of S.cerevisiae wild-type(WT)and adapted strains cultured under the phenolic compound stress were detected using liquid chromatography mass spectrometry(LC-MS)and systematically compared and analyzed by statistical method.The results showed that 565 lipid metabolites were identified,including 185 cell membrane phospholipids.The adapted strain presented the increase in the relative abundance of phosphatidylcholine(PC),phosphatidylethanolamine(PE),phosphatidylinositol(PI),and the phospholipids with long-chain(C32-C36)and diunsaturated fatty acyl chain,while compared with the WT strain.The result of statistical analysis showed that most significant difference in the phospholipids of the WT and the adapted strain were PC and PE with long-chain and unsaturated fatty acyl chain.The mechanism of S.cerevisiae tolerating to phenolics was speculated that the plasma membrane integrity was improved via membrane phospholipids remodeling,i.e.,selectively shielded to phenolic compounds,thus the cells remained high activity.