摘要
基于质谱技术的代谢组学分析,已广泛用于不同生物样本中小分子代谢物的定性、定量研究,以揭示细胞在受到外源性刺激后发生的内源性代谢变化。由于生物样本成分复杂,所含的代谢物极性跨越大,覆盖范围从水溶性到脂溶性,从而需要多种前处理提取方法和与之匹配的不同色谱质谱条件,来准确获取目标成分的定性、定量信息。因此,小分子代谢物结构与理化性质的多样性,在一定程度上制约了生物样本中整体代谢轮廓信息的高通量获取。重点介绍并讨论不同类型代谢物的前处理提取方法研究进展,以及相应代谢物的液相色谱质谱分析条件。
Mass spectrometry(MS)-based metabolomics analyses are now widely applied to identify and quantify small-molecular metabolites in biological samples for uncovering endogenous alterations in cells after their exposure to exogenous stimulus.Duo to the complicated components in biological samples and a big span of polarities of metabolites ranged from hydrophilic to hydrophobic metabolites,a series of extraction protocols and the matched liquid chromatography(LC)and MS conditions are required to obtain the quantitative and qualitative information of target components.To some extent,the diversity of structure and physicochemical properties of small molecule metabolites limits the high-throughput acquirement of whole metabolic profiles from biological samples.Present review focuses on the introduction and discussion of extraction methods of varied metabolites,as well as their corresponding LC-MS conditions.
作者
田鹤
税光厚
TIAN He;SHUI Guang-hou(Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101)
出处
《生物技术通报》
CAS
CSCD
北大核心
2021年第1期24-31,共8页
Biotechnology Bulletin
基金
国家重点研发计划(2018YFA0800901,2018YFA0506902)。
关键词
质谱
代谢组学
脂质组学
mass spectrometry
metabolomics
lipidomics